FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486327
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC37820.7776660559664588No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT34470.7087823624845012No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC32220.6625171952204998No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG25060.5152911518381665No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20090.4130965379261279No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG19900.40918970157939No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG18350.3773181419086335No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC16870.34688594299720144No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA15450.31768748187947615No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC14470.2975364312489333No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA13890.2856102992431019No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC13650.28067534806827504No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT12650.26011305150649666No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT12600.2590849366784077No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11830.24325196832583837No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT11720.24099011570404275No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11370.2337933119074203No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG11260.23153145928562469No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA11030.22680213107641564No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA10830.22268967176405996No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10640.21878283541732207No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10610.2181659665204687No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA10400.2138478842424953No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG10290.21158603162069967No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG10110.20788481823957955No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT10060.20685670341149062No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT9420.19369683361195245No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA8980.18464942312476995No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT8880.18259319346859212No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG8590.17663012746567638No Hit
GTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAG8040.16532086435669827No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC7980.16408712656299157No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC7960.16367588063175598No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA7870.16182527394119595No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG7860.16161965097557815No Hit
CCTAAATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATAC7820.16079715911310702No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC7820.16079715911310702No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA7710.1585353064913114No Hit
GTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCC7690.15812406056007583No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT7530.15483409311019128No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7470.15360035531648458No Hit
CTTTTGGGCAGCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGG7430.15277786345401345No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT7360.15133850269468896No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA7310.15031038786660003No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG7110.14619792855424435No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT7110.14619792855424435No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA7010.14414169889806652No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC6820.1402348625513286No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA6610.13591678027335516No Hit
TGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGG6540.1344774195140307No Hit
ATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTT6380.13118745206414614No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT6340.130364960201675No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC6180.12707499275179046No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG6000.12337377937067036No Hit
GTCGAAGAGCAATACTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTA5970.122756910473817No Hit
CTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGT5960.12255128750819921No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC5950.12234566454258143No Hit
ATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAA5840.12008381192078581No Hit
CCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGT5690.11699946743651905No Hit
TCATTTGGCTGGGAATGGAACATTTACCATATGTATGAGTTACTAATTTC5630.11576572964281234No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA5620.11556010667719456No Hit
GTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATACTCATGCTGTC5410.1112420243992211No Hit
GAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAA5410.1112420243992211No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT5400.11103640143360333No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA5050.10383959763698086No Hit
CAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTG5050.10383959763698086No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA5040.1036339746713631No Hit
ACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACA4870.10013838425586077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1250.098.551251
CGAAAGC1700.072.449243
GCGAAAG1850.066.574982
GTAGAAA8250.061.4847341
TAAGATG9550.053.8683325
TTAAGAT9350.053.470724
AGATGTA10050.050.4777377
AAGATGT10550.049.454216
CGTAATA604.9596553E-448.29953
TAGAAAC10900.046.5270352
CAATTCG6550.045.3639079
ATTAAGA11800.043.5923423
GGTCGTT3550.042.8705257
AGTAGAA7800.042.7352681
GCATTAA13400.042.1806221
GATTAGC6300.041.4080851
AGGTCGT3700.041.12836
TATATTC4800.040.75273
CATTAAG14100.040.592132
ACAAGGT5800.039.9843377
Produced by FastQC (version 0.11.5)