FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_A08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_A08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403622
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG80340.5723763235400984No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG79910.569312820688191No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA79200.5642544787699253No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA78160.5568450765234515No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA67020.4774789793833383No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG64510.4595966720384833No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG62200.4431392497410271No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG58780.4187737154305076No Hit
GATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGG58060.413644129259872No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAT54820.39056099149201134No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG49230.3507354544172149No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT44600.31774936556993266No Hit
ATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA43450.30955627654738954No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC43370.30898632252843006No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC42960.3060653081812625No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT42590.3034292708435747No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG41240.29381129677363277No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC41160.2932413427546733No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT41120.2929563657451935No Hit
GTCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACATCT39810.28362336868473137No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT38790.2763564549429975No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC37650.2682346101728243No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA36990.2635324895164083No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT36500.2600415211502812No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA34060.24265792357201582No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACA33750.2404493517485477No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT33750.2404493517485477No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA33710.24016437473906796No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC31550.22477561622716086No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG31120.22171211337525346No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30360.216297550195138No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC29440.20974307897710354No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG28660.2041860272922482No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG28570.20354482902091875No Hit
CTGTAAGTCTGGCGACCTTGAGTTAGTTTATCTACCCTTAGGAATGTGTA27100.19307192392253755No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC26730.19043588658484975No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC26660.1899371768182602No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG25710.18316897284311587No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG25300.18024795849594835No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA24580.17511837232531266No Hit
CCATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCA24420.17397846428739364No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT24100.17169864821155553No Hit
GCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC23340.1662840850314401No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC23270.16578537526485052No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCAC23190.165215421245891No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA22760.16215191839398357No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC22360.15930214829918596No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC22190.15809099600889698No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA22120.1575922862423074No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA21990.15666611096149818No Hit
ATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAA21940.15630988969964849No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG21880.15588242418542883No Hit
TGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAAT21820.1554549586712092No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC21500.15317514259537113No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC21190.15096657077190298No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG21170.1508240822671631No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC20280.14448334380623842No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA20130.14341468002068933No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG20020.14263099324461997No Hit
ATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAGATCC19940.14206103922566046No Hit
TGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG19770.14084988693537148No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT19770.14084988693537148No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT19710.14042242142115186No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA19190.13671772029791496No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC18750.1335829731936376No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG18460.13151688987490934No Hit
GGATACACCATGGACAGAAAAGGGAAAGTGGACGACAAACACAGAGACTG18440.13137440137016948No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT18390.13101818010831978No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT18280.1302344933322504No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT18250.1300207605751406No Hit
GAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGC18090.12888085253722156No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG18040.12852463127537186No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA17790.12674352496612337No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA17670.1258885939376841No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG17360.12368002211421594No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA17100.1218276715525975No Hit
GGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTA16960.12083025201941834No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG16790.11961909972912936No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA16590.11819421468173055No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT16440.11712555089618144No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA16210.11548693309167282No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA16140.11498822332508325No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC16130.11491697907271331No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG15980.1138483152871642No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG15930.1134920940253145No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA15860.11299338425872492No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA15630.11135476645421631No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC15450.11007236991155739No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC15390.10964490439733775No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA15350.10935992738785798No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATATCCTCAGGTAGTGGT15270.10878997336889847No Hit
CTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCA15210.10836250785467881No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG15030.10708011131201992No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT14880.1060114475264708No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA14740.10501402799329164No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA14630.1042303412172223No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG14350.102235502150864No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG14340.10216425789849404No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT14180.10102434986057501No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT14110.10052564009398542No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG14070.10024066308450565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTGA151.2336887E-4144.89434
AGTAGAA10500.0111.751691
AGCGAAA3850.0103.47381
GAGCGAA607.2759576E-1296.575541
GCAGGTA5200.093.305452
CAGGTAC5350.090.724973
GCGAAAG4850.082.121432
GGTACTG6050.079.038895
GTACAAC1650.079.016361
CGAAAGC5050.078.9000243
CCGCACG406.648173E-572.535065145
GCAGGGT702.8303475E-972.447154
CTAGGAC300.001941788472.444573
GTGTTCC300.001943161772.4316561
TTGACGG300.001943161772.4316561
TGACGGC300.001944810972.4161762
ACTAGGA406.702428E-572.416172
CATACGG406.702428E-572.416172
AAATATA15100.070.053235
GAAAGCA6000.066.409894
Produced by FastQC (version 0.11.5)