FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493889
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC21440.14351802577032163No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20970.1403718750188267No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18820.1259799088151797No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC18620.12464112126135207No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG17000.1137969420753483No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA16380.10964670065848264No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC15510.10382297479933247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3000.057.932191
ATATCGT657.350984E-444.5990649
AGTAGAA10250.038.8569561
CGAAAGC4500.038.6305163
GTAGAAA11350.038.281181
CAATTCG5900.034.394199
ACTGATC8750.033.9578638
GCGAAAG5150.033.7457772
ATAGACA11800.033.1469463
CCTTATA8300.032.280412
GTATTAC12400.031.5356671
GGTCGTT4450.030.941667
AGGTCGT4450.030.9302586
TATACGT3750.030.907524
TAGAAAC14100.030.822223
GAGTCGT950.004736503630.5151469
AGTACCG1204.0780977E-430.1841325
TTATACT10550.028.151844
TACGTGC3950.027.5095926
GGATAGC7300.026.7935855
Produced by FastQC (version 0.11.5)