FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_A01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_A01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT851.1261261261261262No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA781.0333863275039745No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT710.9406465288818231No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT610.8081611022787493No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG570.7551669316375199No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG550.7286698463169051No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG510.6756756756756757No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG470.6226815050344462No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC430.5696873343932167No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG410.543190249072602No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG360.47694753577106513No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG350.46369899311075785No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT340.45045045045045046No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG320.4239533651298357No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT320.4239533651298357No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG300.39745627980922094No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC290.3842077371489136No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT290.3842077371489136No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT280.37095919448860626No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC280.37095919448860626No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC280.37095919448860626No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA280.37095919448860626No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC260.3444621091679915No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA260.3444621091679915No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC250.3312135665076842No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG250.3312135665076842No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC250.3312135665076842No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT240.3179650238473768No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA240.3179650238473768No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG240.3179650238473768No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC230.3047164811870694No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC230.3047164811870694No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACTGGATTCCCAAG230.3047164811870694No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA220.29146793852676206No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT220.29146793852676206No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA210.27821939586645467No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT210.27821939586645467No Hit
GCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAGTGGGT200.2649708532061473No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG200.2649708532061473No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCAC200.2649708532061473No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG190.25172231054584No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG190.25172231054584No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG190.25172231054584No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA190.25172231054584No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG180.23847376788553257No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT170.22522522522522523No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG170.22522522522522523No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA170.22522522522522523No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA160.21197668256491786No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA160.21197668256491786No Hit
CCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCAC160.21197668256491786No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT160.21197668256491786No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC160.21197668256491786No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT160.21197668256491786No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG160.21197668256491786No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG160.21197668256491786No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA150.19872813990461047No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA150.19872813990461047No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG150.19872813990461047No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC150.19872813990461047No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT150.19872813990461047No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA150.19872813990461047No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC140.18547959724430313No Hit
GGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTC140.18547959724430313No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG140.18547959724430313No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGA140.18547959724430313No Hit
GTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATG140.18547959724430313No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT130.17223105458399576No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG130.17223105458399576No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG130.17223105458399576No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG130.17223105458399576No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT130.17223105458399576No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG130.17223105458399576No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG130.17223105458399576No Hit
ATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA130.17223105458399576No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC130.17223105458399576No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC130.17223105458399576No Hit
ATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGT130.17223105458399576No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT130.17223105458399576No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA130.17223105458399576No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT130.17223105458399576No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA120.1589825119236884No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG120.1589825119236884No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA120.1589825119236884No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG120.1589825119236884No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA120.1589825119236884No Hit
ACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACT120.1589825119236884No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGTTTGTGCATACCCACTTGGTT120.1589825119236884No Hit
ATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG120.1589825119236884No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG120.1589825119236884No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC120.1589825119236884No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA120.1589825119236884No Hit
GACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATA120.1589825119236884No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA120.1589825119236884No Hit
TCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTG110.14573396926338103No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG110.14573396926338103No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC110.14573396926338103No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA110.14573396926338103No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA110.14573396926338103No Hit
TTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCT110.14573396926338103No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA100.13248542660307366No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA100.13248542660307366No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG100.13248542660307366No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC100.13248542660307366No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT100.13248542660307366No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT100.13248542660307366No Hit
CCCAAGAGGAATCGTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGA100.13248542660307366No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA100.13248542660307366No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA100.13248542660307366No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT100.13248542660307366No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT100.13248542660307366No Hit
ATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTAT100.13248542660307366No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA100.13248542660307366No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA100.13248542660307366No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT100.13248542660307366No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG90.11923688394276628No Hit
GAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAA90.11923688394276628No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA90.11923688394276628No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA90.11923688394276628No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT90.11923688394276628No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT90.11923688394276628No Hit
AGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATG90.11923688394276628No Hit
ACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCT90.11923688394276628No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG90.11923688394276628No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA90.11923688394276628No Hit
ATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTAC90.11923688394276628No Hit
CAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAG90.11923688394276628No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG90.11923688394276628No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA90.11923688394276628No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT90.11923688394276628No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT90.11923688394276628No Hit
GCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCC90.11923688394276628No Hit
TCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGA80.10598834128245893No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA80.10598834128245893No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG80.10598834128245893No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA80.10598834128245893No Hit
CACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAAC80.10598834128245893No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA80.10598834128245893No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG80.10598834128245893No Hit
GGTATGCACAAACTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAA80.10598834128245893No Hit
TCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGC80.10598834128245893No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT80.10598834128245893No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCATCTCTTGTCTTC80.10598834128245893No Hit
TTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCT80.10598834128245893No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC80.10598834128245893No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAA80.10598834128245893No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAA80.10598834128245893No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA80.10598834128245893No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT80.10598834128245893No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAAC400.005820287454.3752
GTAGAAA400.005820287454.3751
GAAACAA450.00927312248.3333324
AGAAACA450.00927312248.3333323
CGTTTTT200.006001198729.010-14
TAAATTC350.003594396220.71428535-39
TGCCGTC350.003594396220.71428550-54
TTTTTCA350.003594396220.7142852
GCCGTCT350.003594396220.71428550-54
TTAAATT350.003594396220.71428535-39
TTGCCGT350.003594396220.71428550-54
AAGTTAA350.003594396220.71428530-34
GAAGGAC350.003594396220.71428525-29
CAAGTTA400.007778569618.12530-34
CATGAAG500.00135981817.420-24
TCATGAA600.004591078514.520-24
Produced by FastQC (version 0.11.5)