FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755152
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT22100.2926563129012437No Hit
TATGTATAGTGTCCAGGAGGCACCCAGGTGTGAGAGTGAAACTATGGGGT16390.2170423967625061No Hit
GATATGTATAGTGTCCAGGAGGCACCCAGGTGTGAGAGTGAAACTATGGG15900.21055363688370024No Hit
CTACAAGAAGGGGGGCGACTGAGGCTGAGATTCGGGGGGATAATGTCCTA12470.16513231773205925No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA12220.1618217259571583No Hit
CTCTAGTGATCACTTTAGCTATTGTTGTCTTGCCTGCACCATCATCTCTG10840.14354725935970505No Hit
TCTCTAGTCCCTATCCCTTTGCTCTGACATTGTTGATTTCCTCCAGCCAT10640.1408987859397843No Hit
GTGTAGGGTGACCTGACTCCATCCCCACTCCAGAACTTGGCCTCAGCACC9990.13229124732504186No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC9930.13149670529906562No Hit
GTCCAGCACGGTAGCCCCAGGTGGCGACTTAACCAGACTTCGTTAAATTT9200.12182977731635485No Hit
CTCTTGCCCCATGCTGTGGTAGTCTTTGAAACCTGGTATGCATTTTAACA9090.12037311693539844No Hit
CTCCCAGGGGAGCACAGTTCTCCCTGAATGTGGGGGTGTGATGGTGAGAA8850.11719494883149352No Hit
GTCCTGGGGCAGCGTGGGCCGTGCTTTCAAGAACTGTTTGGTCACTGTGT8260.10938195224272729No Hit
CTCTAGTCCCTATCCCTTTGCTCTGACATTGTTGATTTCCTCCAGCCATG7860.10408500540288579No Hit
GTCCAGGGGTGGGACCACAGAGGTCTTTGTGGCGCTGGAGTGTGGCATTT7830.10368773438989766No Hit
GTCCAGGAGGCACCCAGGTGTGAGAGTGAAACTATGGGGTCAGCCAGGCT7670.10156895565396105No Hit
CCCTTGAATCCCTCTCTGGGAGAGAAGAGGTTTCTGTTGTTCGAAGCCAC7650.10130410831196898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.089.5047151
TGGCCCG350.003576659862.0967755
GCGAAAG7250.055.947352
TAGCAGT1457.2759576E-1249.959614
TATACTC1453.8744474E-1044.963654
ATTAGCC657.3622866E-444.58236
TTGCGCA1201.9656E-742.31076145
GGAGCGA1755.2750693E-1141.408828
GGTATAT1401.4280886E-841.38141
GTATTAC5700.040.655411
TAGACCT10200.038.351354
ACCTCTT10500.037.2654727
GCGAGAC1209.531188E-636.26637145
GTATACT1803.2232492E-936.220723
TATACCT800.002044391836.220724
ATCAAAT6450.035.9518478
CAGGGTG5950.035.309935
TAGACAG2900.034.9740455
GACGTAT1051.8483748E-434.5394145
GCATTAG1051.8621076E-434.4959223
Produced by FastQC (version 0.11.5)