FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177143
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT16900.1435679437417544No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC16560.1406795945777191No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG16220.13779124541368382No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16070.13651697372366822No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA14820.12589804297353846No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14760.12538833429753227No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA14090.11969658741546269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.046.8693921
CGAAAGC4600.039.368263
GTATTAC13650.037.1459051
CAATTCG2850.035.5985079
GCGAAAG5050.034.427252
TACATCA14650.032.6367725
ATATAAC3350.032.4347423
TATTACA16600.030.5472742
CCTTATA3600.030.1836152
TTACATC15650.030.0871814
ATTACAT17450.028.6429463
GTATATA21500.027.6261861
TATACTG7100.026.5286985
ACATCAA18250.025.4081736
TATACTC1751.2625453E-424.8378585
TACATAC12000.024.1509976
ATATACT12250.023.6540974
CATACAT12000.023.5522298
GTACTAG3402.5320332E-923.4346961
TAACGAC5650.023.0774633
Produced by FastQC (version 0.11.5)