FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815937
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8650.10601308679469125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.057.2751921
TATACTG6900.035.6932335
TTATACT5850.033.432124
CGAAAGC3700.033.2794883
GTATTAG2404.3655746E-1133.1999551
GCGAAAG3750.032.8377721
ATCGTTG900.003641311532.1978456
CCTTATA5700.030.4901332
GTATTAA3500.028.9745061
CAATTCG3351.8189894E-1228.119949
GTTATAG1050.00774309627.5930753
ATAGACC3357.6397555E-1125.9457283
GGTCGTT2251.3096724E-1025.7598537
GTATTAT4250.025.565741
TATCGTT1550.001817479423.3665375
GTATTAC3152.8901923E-822.9956421
TAGAAAC7500.022.2124253
GTATAGA4951.8189894E-1221.9503821
GGCGGTT2003.116977E-421.7348797
CTATACT3055.3830263E-721.3746364
Produced by FastQC (version 0.11.5)