FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_G09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_G09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6312
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT120.19011406844106463No Hit
TATCTGGGCCTTGATGCAAAGACAAAGAACTGCTTTGTTTCTCCTTGTCG70.1108998732572877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGG100.0069370014144.920649
CTATACT100.0069370014144.920644
GGTCTAT100.0069370014144.920641
GTCTATA100.0069370014144.920642
CTGTTAT100.0069370014144.920648
AAAAAAA1950.0029417627.43182790-94
Produced by FastQC (version 0.11.5)