FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212898
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG15550.12820533960811215No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA13920.11476645191928751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3050.061.7455751
TTATACG1157.177692E-637.8001254
CGAAAGC5650.034.622243
TATACGT1050.007733398627.601235
CCGCATA1358.131847E-426.824572
CGGACGT1100.00969894526.3488046
ACGGACG1100.00970284726.346635
CAGCTCG11100.026.1178959
GTATTAC7700.025.3983421
TATAGAC6600.025.2394812
TATACAG12650.025.2011245
GTATAGG3551.5643309E-1024.4841611
GCGAAAG8000.024.4459061
GTACTAT5000.023.1783391
ATACAGC14400.022.6435036
CGCATAC1600.002184111222.6407013
TAGACAT7650.021.7824254
TATATAC22800.021.2902033
GTATATG6200.021.0287361
GTCTAAT6550.021.0108991
Produced by FastQC (version 0.11.5)