FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243667
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.061.846071
GCGAAAG7050.044.1741142
CGAAAGC8150.037.327823
GTATCGT1306.509402E-427.8658566
GTATATA15750.026.210951
TACACCG1400.001003764225.87445
GTTATAT4403.6379788E-1223.044321
TAACGCG1600.0021844822.6400995
ATATAAC5950.021.912733
TACGCCC1700.00310820721.3057584
GTATTAC5550.020.879281
GTACTAG2857.164075E-620.3298241
TATACTG9550.019.724155
CTATAGG4853.6561687E-1019.4128881
GTATAAT3751.8288665E-719.3133341
TATACAG12900.019.0949065
TTATACC6450.019.09264
TACACTA5356.548362E-1118.9584375
GTATTAT6950.018.7575551
TATAGGG4654.5583874E-918.6903232
Produced by FastQC (version 0.11.5)