FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136938
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.052.082981
GCGAAAG5250.044.1484682
CGAAAGC5650.041.0301363
GTATTAC8850.037.6461371
TATAGAC9500.028.9724332
GCGAAGT1804.7081267E-628.1887259
TATTACA13300.026.6851372
TATACAG8600.025.2766655
TTACATC10500.024.840074
ATTACAT12550.023.666913
TACATCA11350.022.9828365
GTATATA9800.022.1718041
TATATAC12150.022.0610853
GTATTAG5300.021.8650261
CAGCTCG7100.021.4393129
GTATAGA20350.019.2191961
TTACGTT1950.00686829418.5769754
TAGGTAC12550.018.492891145
CCTATAC3953.1491254E-718.3402123
CTATAGT3201.9397416E-518.112554
Produced by FastQC (version 0.11.5)