FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_F08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_F08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555985
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGCTGTGA60551.089058158043832No Hit
GTATCTGACCAACCAGAAAAACTCCAATAGCAAAAACGATCGAAGGAACC39210.7052348534582767No Hit
GTCCAGAGGTCATCCCCCAGCCCTCACCTTGCCAGACTCAGCCTCTTTGA38250.6879682005809509No Hit
TGTAGATACTGAGTAGGCAACCGGAAATTCTAGTCTGGAAGTCTGGGCTG35700.6421036538755541No Hit
GTGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTC34430.6192613110065919No Hit
GAGTGGGGACCTGTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAAT29670.5336474904898514No Hit
ATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGAAAAACTCCAAT27810.5001933505400326No Hit
GTCATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGGTTCAGGGAGA27750.4991141847351997No Hit
GGTTTGGGGTGCCGCGTAGATTTCCAAATGTAGATACTGAGTAGGCAACC27580.4960565482881732No Hit
GAGTAGGCAACCGGAAATTCTAGTCTGGAAGTCTGGGCTGGAGATAAGGT25020.45001214061530437No Hit
CCTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTG24610.4426378409489465No Hit
CCTAATATCAGTATGAAAGGCAAGACAGAAAGAAACCTAGTGAAACAACA23070.41493925195823633No Hit
GATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGA22700.4082843961617669No Hit
GCATTAAAAGATGGTATTCAAAGTCTTGAGACAGTCTGAGATCCTGTAGG22370.40234898423518617No Hit
CTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCAGATCAATCCTCCATC21390.3847226094229161No Hit
ACCTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCT21300.3831038607156668No Hit
GATCTATGCCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTATGGCA20790.37393095137458743No Hit
CTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCA20600.3705135929926167No Hit
GAGTAGGAGTGACGTGGAAGCAGGCATCGGAGCAGGTGCAGGAGGTGGGA20450.36781567848053454No Hit
GTCTGAGATCCTGTAGGCAGTGAGGGTAGAGAGAGAAGAGCCGATGGCCT20030.3602615178467045No Hit
ATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATG19300.347131667221238No Hit
CTTTGATACAGTTGCTCATTCCTCCGCCCAGAAGCTCCTTCTGCCCCAGG18930.34047681142476866No Hit
GGATGGAGTTGTCATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGG17760.3194330782305278No Hit
CCTGTAGGCAGTGAGGGTAGAGAGAGAAGAGCCGATGGCCTCATGGTGCT17540.31547613694614063No Hit
ACCTGGAAGACACTCAGATGTATGGCAATATTATTCGTGGCTGGGATCGG17410.3131379443690028No Hit
CTCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCA16290.29299351601212265No Hit
GTCTTGAGACAGTCTGAGATCCTGTAGGCAGTGAGGGTAGAGAGAGAAGA15890.2857990773132369No Hit
GTATGAAAGGCAAGACAGAAAGAAACCTAGTGAAACAACAAAAACCAACA15790.28400046763851544No Hit
GAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGGGAAAGCTGTGG13580.24425119382717161No Hit
GTTCAGGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGTG13190.23723661609575797No Hit
GCCCTCACCTTGCCAGACTCAGCCTCTTTGATACAGTTGCTCATTCCTCC12860.2313012041691772No Hit
GTTTTCTGCATGATAGGTTTGGGGTGCCGCGTAGATTTCCAAATGTAGAT12560.2259053751450129No Hit
GGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGG12460.22410676547029146No Hit
CTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGA12410.22320746063293076No Hit
GGTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTTGAGACAGT12210.21961024128348788No Hit
CTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCGGC12100.2176317706412943No Hit
GTTGGGAACCATCTGTCAGATCATCCAGCAAAACACGCTGACATCCTTCA12070.21709218773887784No Hit
GGTGTAGGAGGTGGGAACAGGCAGGGGAGCCGGTGTGGGGGCAGGAGTAG12020.2161928829015171No Hit
GCCTAACCTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTA11940.21475399516173999No Hit
CTCAGATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAAC11900.21403455129185142No Hit
CCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTATGGCAATATTATT11710.21061719290988065No Hit
GCACTAATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCA11560.2079192783977985No Hit
TCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAG11510.20701997356043778No Hit
CACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCCCC11510.20701997356043778No Hit
CATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGGTTCAGGGAGAGA11330.20378247614593917No Hit
GCTCTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCTAA11120.20000539582902419No Hit
GTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCAGATCAATCCT10950.1969477593819977No Hit
ATCTTATGCAGTAAATGTTAATGGAGGAGTTGTTCTAGTATCCAGTTTGT10820.19460956680485983No Hit
CTCCCTGCACAGATCCGTCCAGATCCTCAGGGTCCTCCTGGCTGGGCTCA10650.1915519303578334No Hit
AAAGCAGGGCGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAAC10590.19047276455300052No Hit
GATTCAGGGTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACAT10540.18957345971563982No Hit
GTGTAGGAGGTGGGAACAGGCAGGGGAGCCGGTGTGGGGGCAGGAGTAGG10390.18687554520355765No Hit
CCCTTCTCTCCTCCCAATCCTTCTCCGGTTCTCTCAACCCTCTTAATGCT10190.18327832585411477No Hit
GTATTATAGAGGTCACGGATTGCATGGAGCACTGGGTGTGGCGAAAAAAT10190.18327832585411477No Hit
GTGGGGACCTGTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCC9610.1728463897407304No Hit
AGCGAAAGCAGGGCGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCAT9590.17248666780578611No Hit
GCGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGG9510.171047780066009No Hit
TCCCAATCCTTCTCCGGTTCTCTCAACCCTCTTAATGCTCTCACCAGGTC9470.1703283361961204No Hit
TGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCG9420.1694290313587597No Hit
GCTCTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCAT9120.16403320233459537No Hit
GCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCCCCAGCCCTCACC9050.16277417556229035No Hit
GTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCCTTCTCCGGTT9040.1625943145948182No Hit
GCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCTAATAATCAGC8780.15791792944054245No Hit
GTTCTGATGGACCCTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAA8560.15396098815615528No Hit
GTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTTGAGACAGTC8520.1532415442862667No Hit
GTTTGGGGTGCCGCGTAGATTTCCAAATGTAGATACTGAGTAGGCAACCG8510.15306168331879458No Hit
CTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCGGCCAG8470.15234223944890599No Hit
ATCTATGCCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTATGGCAA8470.15234223944890599No Hit
ACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGCTGTGATG8440.15180265654648956No Hit
TTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCCTTCTCCGGTTCTCT8430.15162279557901742No Hit
ATCCTCCATCACGTTTTTCTGTTTTTAAAAAAAACTTCCCAGCCTAACCT8410.15126307364407313No Hit
GTTTAAGGAAGCTGAGCGGCTCTTCAGTAAATCCTCAGTTACTTCAGCAG8210.14766585429463025No Hit
CTAATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCAGAT8140.14640682752232523No Hit
TGGGAAAGCTGTGGGAGAAACAGGGGTTCAGGGAGAGAGCAGGTGCTCAG8100.14568738365243666No Hit
TCTCTCAACCCTCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTT8040.1446082178476038No Hit
CTAATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGC7900.1420901643029938No Hit
ACTCAGATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAA7830.14083113753068877No Hit
GTGCAGGAGGTGGGAACAGATGCAGGAGCGGGTGTGGGGGCAGGAGTAGG7830.14083113753068877No Hit
AATATACATACCTGTGTCGGTTTTTATTCTTTCGTTTTTTATGGCTGCTG7830.14083113753068877No Hit
GGCATAACTTGAATTTTCGGCCAGAGTGGGGACCTGTATTTCCAATTCTT7770.1397519717258559No Hit
GAGTTGTCATTAACAGAGATGGGAAAGCTGTGGGAGAAACAGGGGTTCAG7720.13885266688849518No Hit
AAGCAGGGCTCTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGT7680.13813322301860662No Hit
GGCGGAAACACTGGCCAACTTGGAGAGACAGATCTATGCCTTTGAAGGAA7650.13759364011619019No Hit
GCTGTGATGACTCCCTGAGGAAGTAGAAGAAGCAGCCTTCTGAGGCTTCA7600.13669433527882946No Hit
ATACTGAGTAGGCAACCGGAAATTCTAGTCTGGAAGTCTGGGCTGGAGAT7570.13615475237641306No Hit
GGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCCCCAGCCC7470.1343561427016916No Hit
GTAATGTCCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGGAA7430.133636698831803No Hit
CTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCTAATAA7410.13327697689685872No Hit
CGTTTGGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGGG7340.13201795012455372No Hit
CTTCAGATCAATCCTCCATCACGTTTTTCTGTTTTTAAAAAAAACTTCCC7320.13165822818960943No Hit
ATGCAGGAGCGGGTGTGGGGGCAGGAGTAGGAACAGTCACGGGAACAGGT7180.1291401746449994No Hit
CTCTCAACCCTCTTAATGCTCTCACCAGGTCAGCCATGGCCTCCATGTTG7110.12788114787269442No Hit
AGACAGATCTATGCCTTTGAAGGAAGCTACCTGGAAGACACTCAGATGTA7080.127341564970278No Hit
GAGTAGGAACAGTCACGGGAACAGGTGTGGGAGCAAGTGTGGCCGCAGAG7050.12680198206786153No Hit
GGAGATAAGGTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTT7010.12608253819797297No Hit
GAACATGTTGGGAACCATCTGTCAGATCATCCAGCAAAACACGCTGACAT6870.12356448465336296No Hit
GAGCAGGGAGCCCGATGTGGGACTTGATCCCAGGTCCCTGGGATCATTAC6830.12284504078347437No Hit
CAACTACACCGTGTGTGTGAACCGCCAGCCACTTCTCCAGCTGAAGGAGG6730.12104643110875293No Hit
CCTCTTAAGAAAATAAAATTCTTCAATCAGAACATGTTGGGAACCATCTG6710.12068670917380865No Hit
ACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTTCGGCCA6660.11978740433644794No Hit
GCATGATAGGTTTGGGGTGCCGCGTAGATTTCCAAATGTAGATACTGAGT6590.11852837756414292No Hit
GGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGTGCCGCGT6520.11726935079183791No Hit
CCCTGAGGAAGTAGAAGAAGCAGCCTTCTGAGGCTTCACTCCCTGCACAG6500.11690962885689363No Hit
AAACAGGGGTTCAGGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGT6460.11619018498700505No Hit
GGGCTCTACAGCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCT6450.11601032401953289No Hit
GGTGTGGGGGCAGGAGTAGGAACAGTCACGGGAACAGGTGTGGGAGCAAG6400.11511101918217218No Hit
AGCGAAAGCAGGTGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAA6370.11457143627975576No Hit
GGCCTGAGTGACGGGAAGGATGGAGTTGTCATTAACAGAGATGGGAAAGC6350.11421171434481145No Hit
GAGATAAGGTTTAGGGCCATCAGCATTAAAAGATGGTATTCAAAGTCTTG6350.11421171434481145No Hit
GTGTGGGGGCAGGAGTAGGAGTGACGTGGAAGCAGGCATCGGAGCAGGTG6340.11403185337733932No Hit
GAAAGAAACCTAGTGAAACAACAAAAACCAACATTAAAGTCAATTAACTT6340.11403185337733932No Hit
GTTTTTATTCTTTCGTTTTTTATGGCTGCTGTGATGACTCCCTGAGGAAG6240.11223324370261786No Hit
GCACAGATCCGTCCAGATCCTCAGGGTCCTCCTGGCTGGGCTCATTCTCC6230.11205338273514573No Hit
CTCCCTGAGGAAGTAGAAGAAGCAGCCTTCTGAGGCTTCACTCCCTGCAC6220.11187352176767358No Hit
CTTCTGCACTAATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAA6180.11115407789778502No Hit
GCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGAAAAACTCC6170.11097421693031287No Hit
CTGATGGACCCTATTTGACAGATTCAGGGTAATGTCCTCTTAAGAAAATA6110.10989505112548No Hit
TGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCAGAAAAACT6080.10935546822306358No Hit
GGTTCAGGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGT6030.10845616338570284No Hit
GTTCAGGATCAGCTCATTGAAAAGAGGGAACCAGGAAGTGGGACAGAAAG5990.10773671951581427No Hit
GAGCAGGTGCAGGAGGTGGGAACAGATGCAGGAGCGGGTGTGGGGGCAGG5970.10737699758086999No Hit
GCCTCATGGTGCTCAGTGCGAGTAGGGAGGCTAGAGGAAGACCAGACAGC5970.10737699758086999No Hit
GGAGTAGGAGTGACGTGGAAGCAGGCATCGGAGCAGGTGCAGGAGGTGGG5960.10719713661339784No Hit
AAGCAGGAGCTGGCGGAAACACTGGCCAACTTGGAGAGACAGATCTATGC5920.10647769274350927No Hit
AACTTGAATTTTCGGCCAGAGTGGGGACCTGTATTTCCAATTCTTCTCCT5890.10593810984109284No Hit
CTCTTAAAGCATGTGAAATTCTTCTGTGGGTATTTTGTTGCGAAAGAATC5880.10575824887362069No Hit
GGGAGAGAGCAGGTGCTCAGTTTTCTGCATGATAGGTTTGGGGTGCCGCG5870.10557838790614855No Hit
ATGTGGGACTTGATCCCAGGTCCCTGGGATCATTACCTGAGCTGAAGGCA5820.10467908306878781No Hit
CTTCTCTCCTCCCAATCCTTCTCCGGTTCTCTCAACCCTCTTAATGCTCT5800.10431936113384353No Hit
CTCACCAGGTCAGCCATGGCCTCCATGTTGCTAAGTCCAGAGGTCATCCC5770.10377977823142712No Hit
GCTCTTCAGTAAATCCTCAGTTACTTCAGCAGCTGCAGTAAGTGCGTTGG5760.10359991726395497No Hit
CAGATGTATGGCAATATTATTCGTGGCTGGGATCGGTATCTGACCAACCA5700.1025207514591221No Hit
GGTGCTACTGCAGGTCTTTTGTAAAAATGAAATGGCATAACTTGAATTTT5690.10234089049164996No Hit
CCTGTATTTCCAATTCTTCTCCTCCCTTCTCTCCTCCCAATCCTTCTCCG5650.10162144662176138No Hit
GATCAATCCTCCATCACGTTTTTCTGTTTTTAAAAAAAACTTCCCAGCCT5630.10126172468681709No Hit
ATGTTGCTAAGTCCAGAGGTCATCCCCCAGCCCTCACCTTGCCAGACTCA5600.10072214178440066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4000.0128.499791
CCACCGT203.855746E-4108.816536145
GCCCTCG259.3617546E-487.05323145
ATACCAG259.4054226E-486.9513248
GCACGAT353.448559E-582.795876
CGAAAGC6350.080.981173
GCTCTTA607.991184E-872.54436145
TTGCCAC607.991184E-872.54436145
CACGATG406.685035E-572.446397
ACGGACG502.3162793E-672.4398655
GCACGGA554.083633E-665.842573
TCCCGCC451.18978474E-464.48387145
GTGCTAC7550.064.24391
GTATTGT1250.063.7069321
GACACCC702.3227767E-762.180874145
ACGGCTG606.8205863E-660.3882989
TAGAGGC851.3162207E-859.6348842
TGTGAGG1350.059.0250745
AGCACGA502.0177214E-457.9518935
ACATCGA502.019518E-457.941463
Produced by FastQC (version 0.11.5)