FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837334
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATACAGGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAAA75770.9048957763568659No Hit
GCCCAGGGCAGCCCTGTCCTCCAGGCAATGGGAGAGTGTGAGCGGATGCA33500.4000792992999209No Hit
CGTCAGCACCTTTCTGTGGCCAGGCTTGGGGAAGGAGTACGATTTGATTC30710.36675926213434545No Hit
GGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGA24130.2881765221524505No Hit
GTTATAAACCTTAACAGGAAAAATAAAACAACTTTTACTGGCCATTCCTG23070.27551729656266194No Hit
GATCAGGTCATACCAAAATAAAAACAGGAAAAGAAAAAGTTAGAACACGA20520.245063499153265No Hit
GCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTC18580.22189472779082184No Hit
GTATAACACGGGGACGGAGGTGGGATACCAGCACTTCTCCAGCATGGGCA17920.21401256846133085No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC17910.2138931418048234No Hit
GAATACATACAGTAGGTAGGCTAAAACAGAAGAGTCTTTAGAACTAGTGC17820.21281830189625647No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG17430.20816066229246635No Hit
GGCTTGGGGAAGGAGTACGATTTGATTCTGGAAATTATTCCTCCCGCCGA16710.1995619430239307No Hit
GAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAAAGCCA15960.19060494378587278No Hit
CCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAG15860.1894106772207984No Hit
TCTCCAGCATGGGCAAGTAGGCAGTTGCTGACGGCGGGATCAGATAGAAG15740.18797755734270913No Hit
CTTTTGGACTAAGACAAAAACATTGCCATTACTGTTGAAATTTTGCATTT15440.18439475764748595No Hit
GAACTGTGAGAACTCTCAGCAGTCCATCGGGTTTGTAAGAGTAATGCTCC15410.18403647767796363No Hit
GGGCAGCCCTGTCCTCCAGGCAATGGGAGAGTGTGAGCGGATGCAGTCGG15240.18200622451733717No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15220.1817673712043223No Hit
GGCTAAGGCCACGAGGGTACGGGATCACAGAGCCTGTGTGTCTTGCTCTG15210.18164794454781485No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA14690.17543775840942802No Hit
ATAATAATCAAGGGTTACAGAAAGAGACTTTATCTCAGATTGAATACATA14610.17448234515736852No Hit
GTTCCAGTCACCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTT14400.1719743853707123No Hit
GGGCAGAGGAGTCGATAGCTGGATGGGAAGGCAAGGGAGAAGGGAGTCTC14380.1717355320576974No Hit
GGTCATACCAAAATAAAAACAGGAAAAGAAAAAGTTAGAACACGATCGGC14340.17125782543166765No Hit
GTGCAGAGACTGTCCCTGTGCTGTGGGTCCCTGTGCTGGTGGCCTGACCG14140.16886929230151887No Hit
GGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAA14010.16731674576692218No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG13970.1668390391408924No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13690.16349509275868412No Hit
GGTATAACACGGGGACGGAGGTGGGATACCAGCACTTCTCCAGCATGGGC13640.1628979594761469No Hit
GAGCTAGACTCGGTTTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCT13420.16027057303298325No Hit
GAGTACGATTTGATTCTGGAAATTATTCCTCCCGCCGACCACGTGGCAGG13390.15991229306346094No Hit
TTTGTACCCCGTCTTGCTTTTCTTATCTTTGGTGCCCAATAATAATCAAG13210.15776261324632704No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT13190.15752375993331214No Hit
GTCCCTGTGCTGTGGGTCCCTGTGCTGGTGGCCTGACCGTGCAGCCTCTC12260.1464170808781203No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA11760.14044574805274837No Hit
ATCAAGCATAGTGCATTTTGAAAAGCTGGTGCAGGGCTAGCTGGACGAGT11640.1390126281746591No Hit
TTCCTGAGTAGAGTGCTTACCAAATGCCCCACACCACGCTCTGGCAGCCA11460.1368629483575252No Hit
GCAGGGGACACGTCAGCACCTTTCTGTGGCCAGGCTTGGGGAAGGAGTAC11430.13650466838800288No Hit
GCCCCACACCACGCTCTGGCAGCCAGTGGAGCCGTAGTGGCTGGATTGTG11400.13614638841848054No Hit
GCTTAAAGGCCTAACCAGCTCTTGGCACAGCAAGATCCTTTCTGCAGGCT11350.13554925513594335No Hit
CCCCCGGGCCGTGGCTGTGGCCCACCCGCCACACAGCATTTCCTCGTCAG11230.1341161352578541No Hit
TTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCT11220.13399670860134666No Hit
AAGCAGGGCAGAGGAGTCGATAGCTGGATGGGAAGGCAAGGGAGAAGGGA11040.13184702878421276No Hit
GTGCTGAAGAAGTACGAGCTCCGGGTTAATTACCACATCGTGGACGTCAA10860.12969734896707885No Hit
GACAAAAACATTGCCATTACTGTTGAAATTTTGCATTTTTGTACCCCGTC10860.12969734896707885No Hit
AAAGCAGGGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAA10830.12933906899755654No Hit
GGTTATAAACCTTAACAGGAAAAATAAAACAACTTTTACTGGCCATTCCT10800.1289807890280342No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA10670.1274282424934375No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10650.12718938918042264No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10570.1262339759283631No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT10520.12563684264582592No Hit
GGACACGTCAGCACCTTTCTGTGGCCAGGCTTGGGGAAGGAGTACGATTT10490.1252785626763036No Hit
GTAAAAAGGTTATAAACCTTAACAGGAAAAATAAAACAACTTTTACTGGC10350.12360658948519945No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT10350.12360658948519945No Hit
GTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCTAACCA10260.1225317495766325No Hit
GGGATGGACAGCTTGCCCGTCTTGTCTTTGTCGATCCGGTAGTGAAGCAC10250.12241232292012505No Hit
AATTACCACATCGTGGACGTCAAGGGTGAAAACATCACCAATGCCCCTGA9990.11930722985093166No Hit
GAGCAAGGGGGCTGAGATCTTGTCTGGGTTCATGAAACTGGTGAGGGCTG9800.1170381233772903No Hit
GGATGGCAGGGGCCTCGGGCTAAGGCCACGAGGGTACGGGATCACAGAGC9680.11560500349920104No Hit
ATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCTAACCAGCTCT9590.11453016359063409No Hit
GTGTGAGCGGATGCAGTCGGGCCCTGGGGAGCAGGGCTGCGGTCCGTGGT9580.11441073693412665No Hit
TTACCACATCGTGGACGTCAAGGGTGAAAACATCACCAATGCCCCTGAGC9580.11441073693412665No Hit
TACCAGCACTTCTCCAGCATGGGCAAGTAGGCAGTTGCTGACGGCGGGAT9490.11333589702555968No Hit
GAGTAATGCTCCACCAGCTGCCAGAGCGTGTCGAACTTCTTCCCGTCGGG9480.11321647036905225No Hit
GGCCTAACCAGCTCTTGGCACAGCAAGATCCTTTCTGCAGGCTAATGCCC9450.11285819039952995No Hit
CCCGGGAGGGGCGCGCGGGCTCCGAAGTTGATGTTTCCTGTCTGCCCGCC9420.11249991043000762No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA9330.11142507052144066No Hit
GCATATGCCTGACCCTTCTGATGCCAGAAAGCCATGGCCCTTGGTGTGTG9320.11130564386493322No Hit
GGGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATA9220.11011137729985884No Hit
GTCCATCGGGTTTGTAAGAGTAATGCTCCACCAGCTGCCAGAGCGTGTCG9190.10975309733033652No Hit
GTATATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCG9150.10927539070430678No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC9110.108797684078277No Hit
CTGCCAGAGCGTGTCGAACTTCTTCCCGTCGGGGATGGACAGCTTGCCCG8990.10736456420018775No Hit
GCTTAGCTTAAAGGCCTAACCAGCTCTTGGCACAGCAAGATCCTTTCTGC8960.10700628423066542No Hit
CCTTTCTGTGGCCAGGCTTGGGGAAGGAGTACGATTTGATTCTGGAAATT8960.10700628423066542No Hit
GGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAAAATAAAACAAC8950.10688685757415797No Hit
GTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTCGGTTTGCAGTTC8940.10676743091765054No Hit
CCTTAACAGGAAAAATAAAACAACTTTTACTGGCCATTCCTGTTGAAATG8840.10557316435257616No Hit
GGTCAGGAAGGCCGCTGCTACCTGCGGGGTGCGAACATGATGCGTGGTGT8830.10545373769606872No Hit
ACTCCATCCTGTGTGCAGAGCCCGACCTGGGGCTCAGTCCCACAACTCTG8830.10545373769606872No Hit
GGAGAAGGGAGTCTCTGGGGCATGAGCAAGGGGGCTGAGATCTTGTCTGG8770.10473717775702407No Hit
GAGGTGGGATACCAGCACTTCTCCAGCATGGGCAAGTAGGCAGTTGCTGA8710.10402061781797944No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT8580.10246807128338274No Hit
CTCTGGGGCATGAGCAAGGGGGCTGAGATCTTGTCTGGGTTCATGAAACT8570.10234864462687529No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG8400.10031839146624882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4950.0127.233861
ACGGCGC203.858491E-4108.80198145
GCGAAAG6500.096.893482
GAGCGAA607.2759576E-1296.522231
GGTACTG4250.090.3090445
AGTAGAA3100.084.067761
CTAATAC14450.083.1776663
TAATACA14750.081.4859164
CGAAAGC7750.081.280073
ATACAGG15300.078.111456
GGTGCGC755.4569682E-1177.37029145
TCTAATA16500.072.391682
AGGTACT5850.069.310444
TGGACTA5450.067.766965
GGACTAA5700.064.8063356
CCAATGT451.1965789E-464.413578
TAGCGCC1700.063.943769
TTGGACT6000.061.5439534
TACTGAT6400.059.999537
ACTGATC6600.059.289768
Produced by FastQC (version 0.11.5)