FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114666
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT35550.3189296165846989No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA21510.19297260345251402No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG17040.1528709048270962No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16520.1482058302666449No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA14320.12846897635704327No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC13700.12290677207342827No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT12410.11133379864461641No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC11470.10290077924687754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3600.054.3218571
CGAAAGC4500.043.4516373
ATAGACC18700.033.6924443
TATAGAC18750.033.595062
GCGAAAG5850.033.4168362
TAGGTAC17800.032.59925145
TAGACCT20550.030.6661874
TTACGCA2651.4006218E-1030.0676294
TACGCAG2701.7462298E-1029.5134725
TATACAG14050.028.3581375
GTATTAC14350.027.2555661
GAGCGAA2456.501068E-826.6066251
ATTACAT15550.026.5460153
CCTCTTA22750.026.4401268
CAGCTCG13800.025.2108549
GTTACGC3201.2569217E-924.8941673
AGACCTC25800.024.4281675
TTACATC14600.024.3105954
AAGGTAT8000.023.5468716
ACCTCTT26350.023.3736827
Produced by FastQC (version 0.11.5)