FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122613
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.091.809261
CGAAAGC5600.058.2005163
GTCGAGC604.955686E-448.3128558
GCGAAAG7350.044.341272
TAGCGTA700.001058436841.414719
GTATTAC6650.031.584841
TATATGC2301.0131771E-931.4943434
GTACTAC1652.405517E-630.7267361
GCTTACT2054.0548912E-728.2806958
TACATCA6700.028.1123815
CTATACT3351.8189894E-1228.1098754
CACTCCG2051.2937971E-524.7478169
TTACATC8000.024.4474834
ATTACAT10150.024.2613623
CGTACTA300.001496491424.176908145
GTCTATA6600.024.1424351
TATTACA9400.023.1140672
TATACTA2852.800425E-722.8768795
ATCGGAC1600.002179548122.648679
GTATTAA8200.022.0814951
Produced by FastQC (version 0.11.5)