FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311139
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG19200.1464375630653958No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA14570.11112475488868838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4650.043.617961
CATACCG1406.621158E-736.2433749
TATACAG12650.035.5067675
CAGCTCG10650.030.6282049
ATAGACC5550.028.7126583
CGAAAGC8050.026.9941163
GCGAAAG8100.026.8223692
TATAGAC8850.026.1859052
GTATATA22700.025.5284481
ATACAGC15950.024.984946
ATATATG11150.024.0364633
TAGTACT5300.023.2362734
GTATAGG5350.023.0173551
TATATAC25300.022.3314933
TATATGT11850.022.0078494
TACCGTA1700.003097952321.3180057
AACGACT6150.021.2026844
GTACTAT6250.020.8618471
CCTCGCG1750.00364460220.728704145
GACCGTC2104.3226668E-420.708927
Produced by FastQC (version 0.11.5)