FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973897
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG123861.271797736310924No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA106421.0927233578088853No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA98931.015815840894879No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA85440.8773001662393457No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG84080.8633356504845995No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC81830.840232591331527No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG80630.8279109597832214No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG79620.8175402532300644No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTCT70570.724614615303261No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC65700.6746093272697216No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG65320.6707074772794248No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC64840.6657788246601026No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG58460.6002688169282788No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT56440.5795274038219648No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT56200.5770630775123037No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT54550.5601208341333836No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG51490.5287006736852049No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC50520.518740688183658No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT48920.5023118461192508No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG47650.48927145273062755No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA46200.47438281460975856No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG45040.46247190411306327No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC42400.4353643147067913No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG40070.41143981345049835No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC37950.3896715977151588No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT37150.3814571766829552No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG34830.3576353556895647No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAAGAGGCAATTCT34460.35383618596217054No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA34190.35106381886380184No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC34130.35044773728638656No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC33630.34531372414125927No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA33530.34428692151223383No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT31520.3236481886688223No Hit
GTTCATTGATGCTTCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC30540.3135855229043728No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG29770.30567914266087687No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG28460.2922280282206435No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA27030.2775447506255795No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT26880.27600454668204133No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC26870.2759018664191388No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT26790.2750804243159184No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT26580.27292413879496497No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG25590.262758792767613No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG24660.2532095283176763No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC24420.2507452020080152No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA24260.24910231780157452No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC24020.24663799149191343No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA23460.24088789676937086No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA23410.24037449545485814No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA23390.24016913492905306No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC23350.2397584138774429No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT23100.23719140730487928No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC22350.22949038758718837No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC22270.228668945483968No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA21960.2254858573339891No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA21740.22322689155013314No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT21610.22189204813240002No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC21570.22148132708078985No Hit
CACTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCAAGATTGCTC21300.21870895998242113No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC21270.21840091919371352No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC21220.2178875178792008No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20220.20761949158894627No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG19520.2004318731857681No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC19400.19919971003093756No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG19340.1985836284535223No Hit
CTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAG19110.19622198240676372No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG18920.1942710574116154No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG18140.1862619969052169No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA18040.18523519427619142No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA17940.18420839164716596No Hit
CTTCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGA17650.18123066402299218No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG17550.1802038613939667No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG17500.179690460079454No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC17480.17948509955364889No Hit
CATTTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTG17040.1749671679859369No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT16890.17342696404239874No Hit
GTATATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCG16660.1710653179956402No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCAAG16260.1669581074795384No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC16220.1665473864279282No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA16180.16613666537631805No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA16120.16552058379890278No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG15740.16161873380860606No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG15640.1605919311795806No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA15380.15792224434411442No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT15340.15751152329250423No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA15320.15730616276669915No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG15240.1564847206634788No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC15020.15422575487962278No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA14970.15371235356511007No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT14830.15227482988447444No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA14580.1497078233119108No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA14360.1474488575280548No Hit
ATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATATGGGGAA14190.14570329305871155No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA13650.14015855886197412No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA13640.14005587859907156No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG13640.14005587859907156No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA13490.13851567465553338No Hit
GTATGATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGG13440.13800227334102066No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC13440.13800227334102066No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA13330.13687279044909267No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG13320.13677011018619012No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG13150.13502454571684686No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC13120.13471650492813922No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC12990.13338166151040612No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT12770.13112269572655014No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT12700.13040393388623234No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA12630.1296851720459145No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTCTCTTGTCTTCTTTGCCCAAA12620.12958249178301195No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC12540.1287610496797916No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG12520.12855568915398652No Hit
ATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAA12250.1257833220556178No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT12060.12383239706046945No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG12020.12342167600885925No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA11940.1226002339056389No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG11940.1226002339056389No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG11910.12229219311693125No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA11590.1190064247040498No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATA11530.11839034312663453No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA11340.11643941813148619No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA11240.11541261550246074No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT11230.11530993523955818No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT11150.11448849313633783No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT10980.11274292866699455No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG10980.11274292866699455No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT10870.11161344577506657No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT10830.11120272472345638No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC10810.1109973641976513No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATAT10780.11068932340894365No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC10770.11058664314604111No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA10770.11058664314604111No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG10720.11007324183152838No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG10650.10935447999121056No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT10590.10873839841379529No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCT10350.10627407210413423No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT10330.10606871157832912No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA10320.10596603131542658No Hit
ATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGAT10280.10555531026381638No Hit
GGATACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGAC10200.10473386816059603No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC10170.10442582737188841No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC10160.10432314710898587No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA10150.1042204668460833No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA10020.10288562342835023No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT9880.1014480997477146No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCAAGA9850.10114005895900696No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA9840.10103737869610441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT100.0070831017145.05292145
CACCTTC100.0070831017145.05292145
TCAACGG100.0071027223144.918783
CCGCACA700.0113.97015145
AGTAGAA15400.0112.958541
GCAGGTA11450.0112.644292
AGCGAAA2700.0110.064851
CAGGTAC11850.0108.841953
CCGCACG203.8604173E-4108.78969145
GGTACTG12600.0102.379045
TAGATAT301.6038943E-596.612513
GGTCAAA13400.092.495341
GCGAAAG3650.087.34832
GCCGCCC502.2628228E-887.03175145
CCTGGGG259.373074E-487.03175145
GGCGAAA259.396074E-486.978081
CCTCGCC259.4075897E-486.951262
GTCAAAT14250.086.951262
GACGCCC353.4310135E-582.88738145
AAATATA15250.081.737285
Produced by FastQC (version 0.11.5)