FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822424
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT78210.9509693296888224No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA41730.507402507708919No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC41080.49949904185675514No Hit
TCTATATTCTATACGTGTAAAGCTCACACTATGTTGTTAGGCAAATTTAT34670.4215587093761855No Hit
CCTCTAGAGAGGTAATATCGAAGCAGAAATCTGAATGGCATGAGGCATCC29720.3613707771173993No Hit
GTGCAAGGCACCCAGCCTCGGAGTAGCTGTGTCCCCTTCCGAGGGACTGC22560.274311060961256No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACG22100.2687178389735708No Hit
GCTCTAGATTCCTGAGCTCGCTGCCTGACCTCCCACATTCTGACCTTAGA20460.24877678666964972No Hit
GTCCCAGGACCCTGGGATCATGACCTGAGCTGAAGGCTTAACCGACTGAG18940.23029483575382043No Hit
AAAGCAGGGAGCCCAAAGGGTAGTTTATATGAGAAAGGAGGGGAATGAAA18210.2214186356429287No Hit
GTTTAAGACCAGCATCCAAGCCAGTGTACCTAGAGTCAGGGACCTGGGCT18150.2206890849488828No Hit
GCCCAAAGGGTAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAG18150.2206890849488828No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC17240.20962423275585346No Hit
GAATATAGATGTAGATGTATGTGTAGAATACAGAATACGTGTAGAAATAA17210.20925945740883048No Hit
GAAAAGGGAGATCCCATCTTGTCACACCTCTTCCCGTGGGGCAGGCGAGG17160.20865149849712558No Hit
GTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATT17030.20707080532669278No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT17000.20670602997966986No Hit
CATCTACATCTATATTCTATACGTGTAAAGCTCACACTATGTTGTTAGGC16330.19855938056282402No Hit
GAGTAGCTGTGTCCCCTTCCGAGGGACTGCTCTAGATTCCTGAGCTCGCT16000.19454685174557163No Hit
GTCTTAATGCAGCTTACATAATGCAGATAGCTGTTAGTTTAACAGCTACC15960.1940604846162077No Hit
GTCTTATAGGGGTTCAGAGAAAATACCTACAAATCATACACATGAGCCAA15840.19260138322811593No Hit
GGGTAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAA15840.19260138322811593No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG15360.18676497767574876No Hit
GCACTGGAACATGTTAGCAACACCAGCCGTGGGCTCGGGAGCCCAGAGTG15170.1844547338112701No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA14990.18226608172913242No Hit
GGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGG14730.17910469538826687No Hit
GTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAGGTCCA14670.178375144694221No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA14590.17740241043549312No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT14470.17594330904740135No Hit
ACCTTAGACAGCAGCCACATGGGTTCCTCCCAAGTCGTGCATAGGAGAAT14330.1742410240946276No Hit
GACATAAGCTGGAATGTGAGGATTTCTGAAACGCGTGGCACTGGAACATG13780.16755347606587356No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC13680.16633755824246374No Hit
CCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAG13660.16609437467778176No Hit
ATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGGCTGTACGGAAAGGC13610.16548641576607687No Hit
TGTCACACCTCTTCCCGTGGGGCAGGCGAGGCAGGAGACATAAGCTGGAA13410.16305458011925722No Hit
GGTCAAGAAAGAAAAGGGAGATCCCATCTTGTCACACCTCTTCCCGTGGG13400.16293298833691625No Hit
GCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAG13220.16074433625477855No Hit
GATCTGAGCAGCGAATGATGTGATCTATTTCTCAGTGTTTTTACCCTTTG13000.15806931704327695No Hit
GTATAGATTTTTTAATTCTTTGGTTATGTTTATTCCTAGGTACTTGCCAT12980.15782613347859498No Hit
ATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTG12930.15721817456689008No Hit
GTGTATAGATTTTTTAATTCTTTGGTTATGTTTATTCCTAGGTACTTGCC12800.1556374813964573No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG12540.15247609505559176No Hit
GTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTAC12440.15126017723218194No Hit
ATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGACA12440.15126017723218194No Hit
AGAAAAGGGAGATCCCATCTTGTCACACCTCTTCCCGTGGGGCAGGCGAG11590.14092487573319845No Hit
CCTCTTCCCGTGGGGCAGGCGAGGCAGGAGACATAAGCTGGAATGTGAGG11510.1399521414744706No Hit
GTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTA11440.13910099899808373No Hit
GTATTCTACACATACATCTACATCTATATTCTATACGTGTAAAGCTCACA11340.13788508117467388No Hit
GTGTAAACACTCAAGGGCAGCAGGCTGTTGGTTAAAATACTGAGTCTTAA11330.1377634893923329No Hit
TATATACACTATCTCCTTTGATCATCACACCAATCCATAAAGAAAGGGCA11320.13764189760999193No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA11190.13606120443955916No Hit
ATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTCAATTTC11160.1356964290925362No Hit
TTCTATACGTGTAAAGCTCACACTATGTTGTTAGGCAAATTTATGTCCTG11010.1338725523574215No Hit
GTGTTACAGTTGCCCTAAGTTTTATTTCTACACGTATTCTGTATTCTACA10870.1321702674046477No Hit
GAGCAGGGAGCCTCAGTCCCAGGACCCTGGGATCATGACCTGAGCTGAAG10720.130346390669533No Hit
GATCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGA10700.13010320710485104No Hit
TGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGA10500.12767137145803137No Hit
ACATAATGCAGATAGCTGTTAGTTTAACAGCTACCCTGTGCTCACCAACG10280.12499635224652977No Hit
GTATAGGAAGGAACCGACTTTCCCATATCTAAGGTCACACAGAGAGAGGA10240.12450998511716585No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG10100.12280770016439209No Hit
GGGAAGGACTGGAAAAGATCAGAGGAGAGAGACAGGGAATGGGCCTGAAG10090.12268610838205112No Hit
ATAGTAGGGAGAGCACCAGGCATTAGAGGTCATAAGGCCCCTAATGTGGT9960.12110541521161834No Hit
GGGTAGTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGG9950.12098382342927735No Hit
GTGTAAAGCTCACACTATGTTGTTAGGCAAATTTATGTCCTGGTACTCTC9910.12049745629991342No Hit
ATTCCAGACAGAGGAAAGGGCGTGAGCTTGGTATGTTTAAGACCAGCATC9880.12013268095289048No Hit
CTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTT9720.11818721243543476No Hit
CTCCCAAGTCGTGCATAGGAGAATCTCTTCCCTCCGGCATCCTCTTGATT9710.11806562065309377No Hit
TTCTAGTTCTGTGAGAAGTGTTATTGGTATTTTGATAGGTATTACATCAA9610.11684970282968396No Hit
AGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTCAATTTCTTTCATC9450.11490423431222824No Hit
CCCACATTCTGACCTTAGACAGCAGCCACATGGGTTCCTCCCAAGTCGTG9450.11490423431222824No Hit
CCCAGGCACCCCTCGCTGAGTATTTTTTAGGATTCCATTGGCTCTCCATC9450.11490423431222824No Hit
GTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAA9390.11417468361818235No Hit
ATGTAGATGTATGTGTAGAATACAGAATACGTGTAGAAATAAAACTTAGG9360.11380990827115939No Hit
AGCGAAAGCAGGTCAAGAAAGAAAAGGGAGATCCCATCTTGTCACACCTC9310.11320194935945449No Hit
GGGTGATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGG9290.11295876579477251No Hit
TTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACA9280.11283717401243155No Hit
CATCTATATTCTATACGTGTAAAGCTCACACTATGTTGTTAGGCAAATTT9160.11137807262433977No Hit
CCACTGAGCAGGGAGCCTCAGTCCCAGGACCCTGGGATCATGACCTGAGC9110.11077011371263484No Hit
CTCATGACCCAGCCGCACAGAAGCCGAACCACAAAGCAGAGCCCAAAAAT9100.11064852193029386No Hit
ATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGGT9020.109675787671566No Hit
GAATGGGCCTGAAGCAGAGATTGCTGTTACGAGCCAACATCGCGGCACGC8970.1090678287598611No Hit
GTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGGC8930.10858146163049717No Hit
CTATGTTGTTAGGCAAATTTATGTCCTGGTACTCTCTGTTTCTTTGTAGA8920.10845986984815618No Hit
TTCTAGTGGTTGCTCTCTGGTTTACAGGTGGAACTCATCACAGGCTGCCG8910.1083382780658152No Hit
GATCAGAGGAGAGAGACAGGGAATGGGCCTGAAGCAGAGATTGCTGTTAC8870.10785191093645127No Hit
GAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGAACTCCTTGGAGAA8730.10614962598367753No Hit
CAGCTACCCTGTGCTCACCAACGCCCTGGCAGCCCTCAGCCCTGCCACTT8650.10517689172494966No Hit
GTTTTTACCCTTTGTGTAAACACTCAAGGGCAGCAGGCTGTTGGTTAAAA8630.10493370816026769No Hit
GTCTTCCCGTTTGTTTGTGCCATCTTCAATTTCTTTCATCAGTGTTTTCT8620.10481211637792673No Hit
GTGTTATTGGTATTTTGATAGGTATTACATCAAATATGTAGATTTTGGGT8620.10481211637792673No Hit
CTTCAATTTCTTTCATCAGTGTTTTCTAGTTTTCAGTGTATAGATTTTTT8580.10432574924856279No Hit
CTAGAGAGGTAATATCGAAGCAGAAATCTGAATGGCATGAGGCATCCACT8500.10335301498983493No Hit
TCCCCAGAGAGGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGG8490.10323142320749394No Hit
TAGCAACACCAGCCGTGGGCTCGGGAGCCCAGAGTGGCTGGATAGCATCA8390.10201550538408413No Hit
GTAGAATACAGAATACGTGTAGAAATAAAACTTAGGGCAACTGTAACACA8360.10165073003706117No Hit
ATGTTGTTAGGCAAATTTATGTCCTGGTACTCTCTGTTTCTTTGTAGAAA8360.10165073003706117No Hit
CGTCAGATCCCCAGAGAGGTTTAGGGGGGACATCCAGCCCAGGTCAGGCA8290.1007995875606743No Hit
GGATAGCATCATTGATTCTCATGACCCAGCCGCACAGAAGCCGAACCACA8290.1007995875606743No Hit
GACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCA8270.10055640399599235No Hit
TTTTAATTCTTTGGTTATGTTTATTCCTAGGTACTTGCCATCCCCTCTCC8250.10031322043131037No Hit
CTTTTGTGGTTCCATACAAATTTTAGGATTATTTGTTCTAGTTCTGTGAG8230.10007003686662841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA500.0130.388931
AGCGAAA4200.0124.179931
GACGGCC259.372517E-487.03183145
ATAAGTC259.390671E-486.989459
ACCTATG353.4441746E-582.8218845
TCTAGAG6250.076.513453
TAGGTAA950.076.343704145
CTAATGG801.0004442E-1072.438291
CGAAAGC8050.065.7053763
TCAGGTT451.1973299E-464.4052663
AGGATAA350.003571434662.1201936
CCTCTAG8500.057.9506381
GACTTAT553.2244646E-452.7080466
ACTCCTA450.00962461148.351017145
CTTTAGG450.00966403648.301012
TACATCA18200.048.1800425
ATTACAT18500.046.998443
CTAGAGA10200.046.883244
TTACATC18550.046.8717584
TGGCCGC1255.178663E-946.416973145
Produced by FastQC (version 0.11.5)