FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674398
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACATGCTCTTCAGGGTAACTAACTGGCCAGCCACGTTCCTCCA16010.23739690805725994No Hit
GGTATGAGAAGAGCCTTTGTTCTCGTGGAGGTGGTGTGGGACGAGGCAAA13870.2056649041070703No Hit
GTGCTAATCTGTGAACTCCGTCTCCCGTCTCCCGACTAGAGTGCGGCCAC13210.19587839821589031No Hit
ATCTAATACAGGTCAAAAATGTAAAAAGGTTATAAACCTTAACAGGAAAA13050.1935059119392406No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT12790.1896506217396849No Hit
GGACAGGCGAGAGCGGGCAACTCGTCAAGCGAGCAGCCTTCGGAGAAGGG12550.18609189232471035No Hit
TCTCATACCTGTCTCAGTTCTCTCAGTTCACCCCTTCGCCCTGAATGTCC11500.17052245113419673No Hit
GTATAACCCCAGATCTGTTCATAAGGGCCAGATATGAATAAGAGGGCCAC10620.1574737766126234No Hit
CTTCAGGGTAACTAACTGGCCAGCCACGTTCCTCCACGACAGGGAGGAGG9360.13879044718400707No Hit
GTGCAGAGCCGGGCAAACGTGGGGGATTCACATGCTCGCCATGAACCTGG8940.13256267070780162No Hit
CCCCAGATCTGTTCATAAGGGCCAGATATGAATAAGAGGGCCACCTCTGT7700.1141759020637665No Hit
GTCACAGGGACAGCGGTCACACACTGGCAGGGAAGAGAAAGCAGATGAGC7570.11224825696398862No Hit
GAATAAGAGGGCCACCTCTGTCTCTTGAGGTCATTTCCAGAGAAAGCTTG7480.11091373343337317No Hit
GTACCAGGACTGGGAAGATCGAATGCGGGGATCTGTAAGTCACAGTGACT7480.11091373343337317No Hit
ATCCTAAACAGAGTCAGTGCAGAGCCGGGCAAACGTGGGGGATTCACATG7460.11061717264879195No Hit
GTTCTAGGTGGCCTAAGCCAGGAAAACTAAGGGAACACATCGCTCGGGGT7090.10513079813403954No Hit
GTGTGTGCACAGCTGCGCGGTCAGCCCATCCTAAACAGAGTCAGTGCAGA7080.10498251774174894No Hit
ATCTCATGCTGGGTTTTTGTTCTTTCTGTTTTAAAATAAGACCTAGAAGG7040.10438939617258651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG100.0070833955145.0476145
AGCGAAA2400.0105.6148451
CTCTACA259.428609E-486.899492
GTGGTAC502.3009288E-672.5238145
TAACGCG406.687573E-572.44315
TAATACA2900.072.437734
CTAATAC3550.067.3264853
AACGCGA451.1973434E-464.403436
TTAACGC451.19866294E-464.389094
CGAAAGC4000.063.3736083
GCGAAAG4000.063.3642122
TATAGAC3200.058.8381962
TGGACTA753.7798054E-757.9544835
CTATACT1206.002665E-1154.3282974
CACGGTT450.00962528848.349205145
GTCTATA1351.9281288E-1048.2774932
GGTCTAT1402.746674E-1046.5567471
GCTATAC2350.043.1479873
ATCTAAT4850.041.8104131
ATACAGG5050.041.6071626
Produced by FastQC (version 0.11.5)