FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_B08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_B08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077315
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA18640.17302274636480508No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC18410.17088780904378015No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG16900.15687148141444238No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14230.13208764381819615No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13990.12985988313538752No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC12300.11417273499394329No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT11320.1050760455391413No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11110.10312675494168373No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11070.10275546149454895No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA10920.10136311106779354No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10900.10117746434422616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA2550.056.828957
AGTAGAA7750.051.397161
AGCGAAA2650.049.1931651
CCTTATA6750.045.0654642
TTATACT7900.041.2614254
GTAGAAA10950.040.3453831
GATAGCA4500.040.246366
GGATAGC4650.038.9480935
GACGGTT951.0325511E-438.1352167
CTATAGT951.03424696E-438.124594
GTCTATC2109.094947E-1237.937762
CCCTTAT8450.037.7115251
GGTCTAT2700.037.5528071
CAATTCG5100.036.9491169
ATCCCGA2201.6370905E-1136.221736
TCTATAC850.002753840834.087873
ATATTCA5750.034.0137674
CGAAAGC4050.033.982663
AGGTCGT3500.033.1170046
GCGAAAG4200.032.764432
Produced by FastQC (version 0.11.5)