FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107897
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT30370.2741229554732976No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA24810.22393778483017823No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC23460.2117525365625144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3100.051.423241
TAGTGCG750.001486160338.6523327
GCGAAAG4950.033.6682931
CCTTATA5750.031.4830232
AGAGCGT1402.7522077E-531.0627148
TTATACT7250.030.9731124
CGAAAGC5400.030.848673
GTATTAC15150.029.6536051
TACTCCG1701.0358992E-425.5718155
GTATTAG2001.07017095E-525.3610081
TATACTC2001.0709211E-525.3587155
CCTATAC2901.2036253E-824.9749343
GTACGCT1450.0012337224.9749343
TATTACA18950.024.8375322
TTACATC16800.023.7145334
TATACTG11400.023.5156025
CTAGTAC1550.001819197423.3636463
GAGCGAA3753.092282E-1023.1872081
GACAGTA4700.023.1297177
CTTATAC13500.023.0694393
Produced by FastQC (version 0.11.5)