FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369099
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC18440.4995949596178803No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT12780.3462485674575114No Hit
CTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA10630.28799861283829No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT9610.2603637506468454No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA9250.2506102698733944No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA8040.21782773727373958No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC7520.20373937615653254No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC6530.1769173040295422No Hit
GCTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATA5730.15524290231076215No Hit
GGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGG4510.12218943968962256No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC4420.1197510694962598No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG4250.11514525913101906No Hit
GATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC4140.11216502889468678No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG4100.11108130880874778No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG3790.10268247814272052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC10250.038.8547972
GTATACC750.001489137838.6297843
GTCTAAC750.00149311238.6088451
CACGGTT800.002028224536.274445145
AGCGAAA1406.658138E-736.2056052
GTATACG800.00204995536.1957931
GTTACGC1051.8618643E-434.490883
TATAGGT900.00364226632.1914864
ATAGACC6600.031.8256763
GAGCGAA1601.8948758E-631.6713181
TTACGCA1153.1759834E-431.491674
GCGAAAG2601.1277734E-1030.6272091
TAGACAT6150.030.6211724
TGTTACG1204.0823338E-430.1713372
ATTACAT7450.030.139013
GTATTAC7450.030.1226751
TATTACA8200.030.024162
CTTAACG6300.029.875891
TAACGAC6600.029.6308023
TAGGTAC12150.029.2584145
Produced by FastQC (version 0.11.5)