FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R2_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R2_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136349
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG14890.13103368771389776No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA14540.12795364804298678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4400.046.0860141
GCGAAAG6700.031.3477292
CGAAAGC7050.028.7692343
GTATTAC10300.027.4214971
GTATATA19450.027.18111
CATACAT10400.024.3938858
CAGCTCG10450.023.5845729
TATAGAC13850.023.5312822
ATAGACA10200.022.0151123
GACATCT10250.021.9173726
TATATAC21850.021.880233
TATTACA13950.021.8050942
TATACAG13400.021.6286455
GTCCTAC3457.593735E-820.9915581
ATACAGC14350.020.705356
TAGGTAC13850.020.42525145
TACGCCC2155.080489E-420.2184874
TACATAC12600.020.1302016
TTACATC11600.019.986094
ATATATG10150.019.982573
Produced by FastQC (version 0.11.5)