FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACTTCACGTGGTC31.9736842105263157No Hit
GACTCCGACTTCACGTGGTCGAGTTGCAGACCACGATCCGAACTGAGACC21.3157894736842104No Hit
GTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGA21.3157894736842104No Hit
AACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGG21.3157894736842104No Hit
AACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACT21.3157894736842104No Hit
CGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAA21.3157894736842104No Hit
GTGTACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGG21.3157894736842104No Hit
AAAGCAGGTCGCTTAAACCAACTCAGCATGGCAAAGACAAAGCCAAGTAG21.3157894736842104No Hit
ACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGAGTTGA21.3157894736842104No Hit
TTGAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAG10.6578947368421052No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA10.6578947368421052No Hit
AGCGGCGGAGCATGCGGATTAATTCGATGCAACGCGAAGAACCTTACCAA10.6578947368421052No Hit
GTCCAAACTCATCAATGTATCTTAACATGTCTGGATCCGCTGCATTAATG10.6578947368421052No Hit
TACCTCCCCCGGGGCCGGCGGCGTGGTCGGTGACGCGACCTCCCGGCCCC10.6578947368421052No Hit
AGTCCCCACCATCACGTGCTGGCAACATAGAACGAGGGTTGCGCTCGTTG10.6578947368421052No Hit
GACCCCGGCAGTCTCCTATGAGTCCCCACCATCACGTGCTGGCAACATAG10.6578947368421052No Hit
ACTCATAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGATGACGTC10.6578947368421052No Hit
TTCTTAACTCTTATGAAACTTGATACTCTTAAATCTTCAAATGCACCAGA10.6578947368421052No Hit
GTCTTGGGCTTCACGCATGCTACAATGGCCAGTACAATGGGTTGCGATAC10.6578947368421052No Hit
CTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGT10.6578947368421052No Hit
ACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTAT10.6578947368421052No Hit
CCCTTCTTGTGTCTCTTTATCAAAAAGATTTGTGAATACTCAAGACTTCC10.6578947368421052No Hit
CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG10.6578947368421052No Hit
CTACAATGGCCAGTACAATGGGTTGCGATACCGTGAGGTGGAGCTAATCC10.6578947368421052No Hit
CCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTC10.6578947368421052No Hit
CGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA10.6578947368421052No Hit
GCGTGAAGCCCAAGACATAAGGGGCATGATGATTTGACGTCATCCCCACC10.6578947368421052No Hit
GTAAGGTTCTTCGCGTTGCATCGAATTAATCCGCATGCTCCGCCGCTTGT10.6578947368421052No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT10.6578947368421052No Hit
CTCACGACACGAGCTGACGACAACCATGCACCACCTGTACACCAGCCCCA10.6578947368421052No Hit
GGCTGTGACATGGTGGAATAGGAATGGACCAATAACAAATACAGTTCATT10.6578947368421052No Hit
TAGATAGGAGGAAGCATCATAAAATATTAGCAGACAGCCCGATGCGCGGC10.6578947368421052No Hit
CCCATCTCTGAGCCGATCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTCG10.6578947368421052No Hit
AGCTGTGCCTTCATCTGGGTCTTCAGTTAAAGTGCCAGCATCCTTTCCGA10.6578947368421052No Hit
GTTTTCCTCCTTGTGGGGCTGGTGTACAGGTGGTGCATGGTTGTCGTCAG10.6578947368421052No Hit
ATGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATC10.6578947368421052No Hit
GCATGACAGTAAGGGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC10.6578947368421052No Hit
GCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTAGTGTCGAATA10.6578947368421052No Hit
CCTCACGGTATCGCAACCCATTGTACTGGCCATTGTAGCATGCGTGAAGC10.6578947368421052No Hit
CAGCTGATAGTGAGTGGGAGAGACGAACAGTCGATTGCCGAAGCAATAAT10.6578947368421052No Hit
TCTCTGAGCCGATCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTT10.6578947368421052No Hit
GCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATT10.6578947368421052No Hit
CGTGTTGAACTCATTTTAGAAGGACCTGCATGGTGGGGGTGATATTGGGA10.6578947368421052No Hit
AGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAA10.6578947368421052No Hit
AACTGAGACCACCTTTTTGGGATTAGCTTGACATACACCGGGTCGGCTCA10.6578947368421052No Hit
TCACGTGGTCGAGTTGCAGACCACGATCCGAACTGAGACCAGCTTTTTGG10.6578947368421052No Hit
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT10.6578947368421052No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT10.6578947368421052No Hit
AACGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCA10.6578947368421052No Hit
ATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGT10.6578947368421052No Hit
CGCCAGGTATCACACACAACCGGTTTAGCCGCAGATGGTGGCTAAACCGG10.6578947368421052No Hit
CCTTCCCCCCTGGCAAGCAGGAGACGGCTGAGATGGGTGGTGTGTTTCCT10.6578947368421052No Hit
GGCCCGGGAACGTATTCACCGCAGCGTTGCTGATCCGCGATTACTAGCGA10.6578947368421052No Hit
GTCCCCACCATCACGTGCTGGCAACATAGAACGAGGGTTGCGCTCGTTGC10.6578947368421052No Hit
GGTGTTGCCCGCCGTCTGGATATAGCGCAGCTCGCCTGCGACATGGTGGA10.6578947368421052No Hit
CCATTGTAGCATGCGTGAAGCCCAAGACATAAGGGGCATGATGATTTGAC10.6578947368421052No Hit
GTCTCCTATGAGTCCCCACCATCACGTGCTGGCAACATAGAACGAGGGTT10.6578947368421052No Hit
CATTCACAGTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCG10.6578947368421052No Hit
CGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTATGT10.6578947368421052No Hit
GTTGGGCACTAGGTGTGGGGGACATTCCACGTTTTCCGCGCCGTAGCTAA10.6578947368421052No Hit
GTGTGGGGGACATTCCACGTTTTCCGCGCCGTAGCTAACGCATTAAGTGC10.6578947368421052No Hit
CCCGCAACGAGCGCAACCATCGTTATATGTTGCCAGCACGTGATGGTGGG10.6578947368421052No Hit
GTGTGCGGGTGGTGGATGGTTGGCGGCGGGGGGGGGGGGGGGAGGGGGGG10.6578947368421052No Hit
ACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCAC10.6578947368421052No Hit
TGAGAGAAATGAGCAAGGATAAACTTTATGGAGTAAAATGAATGATGCAG10.6578947368421052No Hit
TTCTGATGTTTTAAATGATTTGCCCTCCCATATGTCCTTCCGAGTGAGAG10.6578947368421052No Hit
CATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTAC10.6578947368421052No Hit
TACTATATGTCAGAACAAATGGAAACTCCAAGATCAAGATGAATTGGGGC10.6578947368421052No Hit
GCTCAGAGATGGGTTTTCCTCCTTGTGGGGCTGGTGTACAGGTGGTGCAT10.6578947368421052No Hit
GGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTGGGGGACATTCCACG10.6578947368421052No Hit
ATCCAATTACAGCAGACAAGAGGATAACGGAAATGATTCCTGAGAGAAAT10.6578947368421052No Hit
GACAATGTCTGCTTTCGGCCGCCACGCCAGCTGAGTTTTGCACAAATGTT10.6578947368421052No Hit
AGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGATGACGTCAAATCATCA10.6578947368421052No Hit
ATTTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.6578947368421052No Hit
CTGTGAGTCTCTTCGTGGCCTTGTTATAGTTGAATACAGGAGAATTGCCC10.6578947368421052No Hit
CGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGG10.6578947368421052No Hit
TTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGCTGATCTG10.6578947368421052No Hit
GCATGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACG10.6578947368421052No Hit
TCCATGCCGTAAACGTTGGGCACTAGGTGTGGGGGACATTCCACGTTTTC10.6578947368421052No Hit
GTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAG10.6578947368421052No Hit
GTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACTTCA10.6578947368421052No Hit
GCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGC10.6578947368421052No Hit
CCCCACCAACACCTGCTGGCAACATAGAACGAGGGATGCGCACGTAGCGC10.6578947368421052No Hit
ACTAGCGACTCCGACTTCACGTGGTCGAGTTGCAGACCACGATCCGAACT10.6578947368421052No Hit
GTAAGAACTCTGTTCCAACAAATGAGGGATGTGCTTGGGACATTTGATAC10.6578947368421052No Hit
GCGTTGCTGATCTGCGATTACTAGCGACTCCGACTTCACGTGGTCGAGTT10.6578947368421052No Hit
AGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTGGGGGA10.6578947368421052No Hit
GGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTG10.6578947368421052No Hit
CCTATGAGTCCACACCATCACGTGCTGGCAACATAGAACGAGGGTTGCGC10.6578947368421052No Hit
GCCAGAGGTGATACCATCACTCGGTCTGATCCTGCATCATTCATTTTACT10.6578947368421052No Hit
GGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAA10.6578947368421052No Hit
GCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGC10.6578947368421052No Hit
GGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTATGTTGCCAGCACGTG10.6578947368421052No Hit
GTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTG10.6578947368421052No Hit
GTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGA10.6578947368421052No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA10.6578947368421052No Hit
GACGGGGGCCCGCACAAGCGGCGGAGCATGCGGATTAATTCGATGCAACG10.6578947368421052No Hit
CGTTCTATGTTGCCAGCACGTGATGGTGGGGACTCATAGGAGACTGCCGG10.6578947368421052No Hit
GTCTAGAATTGACCTGCATGGTACCCGGGCATGCTCGAGCTAGCAGATCT10.6578947368421052No Hit
CGACTCCGACTTCACGTGGTCGAGTTGCAGACCACGATCCGAACTGAGAC10.6578947368421052No Hit
GAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACA10.6578947368421052No Hit
GTTTAGAGCCTATATAGAAGGATACGAGACGAAGGGCTCCATTGATGGCA10.6578947368421052No Hit
ATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTGGG10.6578947368421052No Hit
CACCATCACGTGCTGGCAACATAGAACGAGGGTTGCGCTCGTTGCGGGAC10.6578947368421052No Hit
CCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTGTGGGGGACATTC10.6578947368421052No Hit
CTGTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGAT10.6578947368421052No Hit
GAAATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAG10.6578947368421052No Hit
GTGTCGAAAGAGTGTAACGACCATACATGACTGTTCTAGTCCGAGAGTAC10.6578947368421052No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG10.6578947368421052No Hit
GAAAAACAACAGCAATCTAATAACACCCATAAAATTAAAAAACAAAAACA10.6578947368421052No Hit
ATGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGATCGGCTCA10.6578947368421052No Hit
TAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTA10.6578947368421052No Hit
TCCTCCGAGTTGACCCCGGCAGTCTCCTATGAGTCCCCACCATCACGTGC10.6578947368421052No Hit
GATGTGGACTGGGTCATGTTTTCCTGGCGTATCCACTTGGGCCCGTGTGT10.6578947368421052No Hit
GACATACACCGGATCGGCTCAGAGATGGGTTTTCCTCCTTGTGGGGCTGG10.6578947368421052No Hit
GATTTGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA10.6578947368421052No Hit
TCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCGCC10.6578947368421052No Hit
GATGTATAGTTAGAAGAATGATACACCATTAGAAAGTAGCATGATGGTGG10.6578947368421052No Hit
ATCCGAACTGAGACCAGCTTTTTGGGATTAGCTCCACCTCACGGTATCGC10.6578947368421052No Hit
GCCCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGAC10.6578947368421052No Hit
GCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCT10.6578947368421052No Hit
AAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTAC10.6578947368421052No Hit
GATAGTGAGTGGGAGAGACGAACAGTCGATTGCCGAAGCAATAATTGTGG10.6578947368421052No Hit
TTGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCGCATGCTCCGCCGCT10.6578947368421052No Hit
GCCATACTCAGAAAAGCAACCAGGAGATTGATTCAGCTGATAGTGAGTGG10.6578947368421052No Hit
ACTTCAATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGT10.6578947368421052No Hit
TGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATC10.6578947368421052No Hit
ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC10.6578947368421052No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA10.6578947368421052No Hit
TTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGC10.6578947368421052No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT10.6578947368421052No Hit
AGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCAT10.6578947368421052No Hit
GGCCCGCACAAGCGGCGGAGCATGCGGATTAATTCGATGCAACGCGAAGA10.6578947368421052No Hit
CAACAATACTTTATTTCAGAGCAACCATCAGCACGAACTTTGACCGCTCA10.6578947368421052No Hit
CTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGA10.6578947368421052No Hit
CACCTCACGGTATCGCAACCCATTGTACTGGCCATTGTAGCATGCGTGAA10.6578947368421052No Hit
CGATCCGAACTGAGACCAGCTTTTTGGGATTAGCTCCACCTCACGGTATC10.6578947368421052No Hit
GAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCCTCACATTCACAGT10.6578947368421052No Hit
GACATAAGGGGCATGAAGATTTGCCGTCATCCCCACCTTCCTCCGAGTTG10.6578947368421052No Hit
GGACATTCCACGTTTTCCGCGCCGTAGCTAACGCATTAAGTGCCCCGCCT10.6578947368421052No Hit
GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA10.6578947368421052No Hit
CCCCACCATCACGTGCTGGCAACATAGAACGAGGGTTGCGCTCGTTGCGG10.6578947368421052No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers