FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650513
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAGCTAAAGTGCCAGGAGAAATGACTAGGAAGGTGGGAAACCCTC15560.23919583467202038No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA6510.10007486399195711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2850.066.076583
TATAGAC1250.063.733582
TCCGCGG350.003554576562.192944145
ATATAGC5400.056.334523
ACCCGCG400.00606383654.3434688
GTATATA5600.051.739761
GGTCCGG858.9557034E-751.146798
TATTGGC752.5583468E-548.3053057
GTTCGGG752.5630052E-548.2904431
TATAGCT6450.046.044384
GCCGATT1755.2750693E-1141.404559
ATGACCG700.001060590641.394995
TATATAG7400.041.1058122
CGTATAA1105.293634E-639.510361
AGCGAAA4850.038.831491
GTTACGC1209.622134E-636.2150463
ATAGACC2103.8198777E-1034.4905173
ATCGAGG850.002749090334.097868
TAGACCT2154.9112714E-1033.6910064
ATAGCTA9100.033.4344145
Produced by FastQC (version 0.11.5)