FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162682
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT19000.16341527606000608No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC16650.14320338665258428No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA15680.13486060676952083No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG13560.11662690228282539No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA12930.11120839576083573No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT12830.11034831536051991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2950.058.911411
CAATTCG3300.043.9238179
GCGAAAG4750.036.591812
CGAAAGC5050.034.423963
TATTAGG2455.638867E-1132.515682
TATACAG9150.031.6718435
CAGCTCG8250.031.6251479
ATAGACC8300.030.5443533
TATAGAC19500.027.482952
TAACGAC6050.026.3395483
TAGACCT9400.026.2028264
ACGACTG6100.026.1292725
CTTAACG6500.025.6226881
TATATAC19600.023.6518383
GTATTAG4002.7284841E-1123.5338821
TATATAT11150.023.3826582
GTAGAAA10350.022.3882331
ATAGACA13600.022.3692473
GTATTAC14600.022.3187481
CCTTATA5200.022.2834722
Produced by FastQC (version 0.11.5)