FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785667
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10500.13364440659974264No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA8780.11175217999483242No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA8000.10182430979028012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2350.073.962351
GGTCGTT2900.039.9821247
CGAAAGC4650.038.936273
AGGTCGT3250.037.894066
GCGAAAG4700.036.9764672
CAATTCG4000.036.245359
AGTAGAA6050.029.9262261
CTATACT2252.8434442E-828.9704344
TCGTTTT4050.028.63839
GACAGTA3850.028.234137
GTACCGC1306.510771E-427.8632816
GTAGAAA8950.026.7028881
GTATACG1500.001503447124.140491
TATACTG6350.023.9580335
GTATAGA6350.023.9504051
TAGAAAC10200.022.717572
CCTTATA4505.456968E-1222.5282572
CCATATA4256.184564E-1122.149632
CCTATAC2353.7389993E-521.5723513
ACAGTAT6950.020.8606328
Produced by FastQC (version 0.11.5)