FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234540
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG19980.16184165762146224No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14340.11615662513972816No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA13050.1057073889869911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4400.052.7000051
CGCTATA750.001489965438.6325762
CGAAAGC6100.038.0007933
GCGAAAG6700.035.677472
CTATAGT2950.031.9248644
CAGCTCG10700.027.0887859
TATACAG12300.026.5042255
CTATACT5000.026.080164
GTATAAC2051.2957667E-524.7432961
TATATGT11750.024.6620864
GTATATA21800.023.9325711
GTATAGA8850.023.7447741
CTTATAT5500.023.7073143
ATATATG13050.022.7586173
TATACTG4800.022.6390275
GTATAGG3854.311005E-1022.5857161
ACTATAC3854.3291948E-1022.5783943
GTCTTAT6100.022.5702881
ATATACT12200.022.56484
TATATAC24200.022.4501063
Produced by FastQC (version 0.11.5)