FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494875
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4600.039.3529471
CTAGTAC3550.030.617043
TATACAG13000.030.1019295
TATACGT2354.3221007E-827.7516944
CGAAAGC6450.026.9619853
GTATTAC12350.026.3839971
GTATTAG6100.026.1148741
GCGAAAG7300.025.796562
GTATAGG6450.023.5751611
GTATATA24900.022.973271
GTAACGA2252.64263E-522.566633145
TATAGAC11450.022.1398092
TATACTG8250.021.0814865
CAGCTCG11500.020.1703019
ATTACAT13450.019.9333233
TCGCGCC2556.970007E-519.8983979
TATTAGG4803.1832315E-1019.6161352
CCTTATA6000.019.3143482
CAATTCG5657.2759576E-1219.2443439
CTATACT7750.018.7000684
Produced by FastQC (version 0.11.5)