FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917922
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2005.456968E-1239.840081
CGAAAGC4050.030.4037932
GCGAAAG4450.029.3001631
CTATACG1050.00768981427.63251145
TATACTT7800.026.008525
AGGGGCG3151.0422809E-925.3008085
CATATAT8650.025.122491
TATATAC12600.024.7217463
GTATATG4201.8189894E-1224.1455081
TATAGAC6500.023.401292
TTATACT4053.274181E-1123.2562964
TATACAG7650.022.7301375
CTAATAC2653.8063754E-621.8688183
GTACATA7050.021.5768341
ATATACT8750.021.5286874
GTGCATA2704.4780645E-621.4626711
CAGCTCG5101.8189894E-1221.31889
GTATTAC7300.020.8379021
GTATAGT5353.6379788E-1220.3093031
ATACTTA7900.020.1776926
Produced by FastQC (version 0.11.5)