FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_F08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_F08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997569
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAAAAT62200.6235157668291618No Hit
GATTAATAAACAGCACTTCAAAATTAACCGCATGAGGGCTGTGCTTGCAG45970.4608202540375654No Hit
CATGAGGGCTGTGCTTGCAGCAGGGTTTCACAAGACAAGGCACCCAGATT45930.4604192792679002No Hit
ATCATACACTAAGATTAATAAACAGCACTTCAAAATTAACCGCATGAGGG43650.43756371739699207No Hit
CTATAGAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCATGCT43330.4343559192396716No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA37390.37481116594441083No Hit
CTCCTATCCGGACACTGATGTTATACTGATGTGTTTCTCCATTGACAGCC37140.3723050736340043No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA34070.3415302600622112No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC33790.33872343667455584No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC32090.321682008963791No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT31980.32057932834721203No Hit
CTCTATAGAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCATG31720.3179729923443892No Hit
ACTAATACCTGGTCAGCCTTCTCATGTTCACTTGTAGAAATGAGCATCTC29630.2970220606293901No Hit
GATGTTATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAAAA28930.29000500216025155No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC28480.2854940360015197No Hit
GTAGAGAGGCAGGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAGCAGAG25860.25923018858845853No Hit
GATTATGACCGCTTGAGGCCTCTCTCCTATCCGGACACTGATGTTATACT25200.2526141048889851No Hit
CTTTAGTGCAGGTACTTTAAGAAGGAAGACAAGCAGGTGCTATGAATCTC22910.22965829932566068No Hit
ATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAAAATATCCC22210.2226412408565222No Hit
CCCTGATAGTTTAGAAAATATCCCAGAAAAATGGACCCCGGAAGTCAAGC21410.2146217454632211No Hit
GGTTTTAGGAGAGTACTTCGTTCTCTTGCCAGAACGACACTTGTCAATAA21350.2140202833087235No Hit
CACTAAGATTAATAAACAGCACTTCAAAATTAACCGCATGAGGGCTGTGC21040.2109127288438193No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG20730.20780517437891513No Hit
ATTATAGATAAATGAAAAAGGCCAGTAATCATACACTAAGATTAATAAAC20660.2071034685320013No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG20560.20610103160783863No Hit
GTAATCATACACTAAGATTAATAAACAGCACTTCAAAATTAACCGCATGA20260.2030937208353507No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT20010.20058762852494413No Hit
TCACAAGACAAGGCACCCAGATTTTTTCTTCCCACGTCTGGCTTGCAGAG18570.18615253681700214No Hit
TCTAAATACTGGTAGCAAGATGACTTCTGATTTGTAATCTTAGGTAAATT18540.18585180573975332No Hit
GGCCAGTAATCATACACTAAGATTAATAAACAGCACTTCAAAATTAACCG17830.1787345035781986No Hit
GTACAGGGTCTGATGAATGAGGGACGGACCAGAATTTGTTTAACAGTGGA17500.1754264617284619No Hit
CTTCTGTCCCAACGTGCCCATCATCCTGGTTGGGAACAAGAAGGATCTTC17380.17422353741946672No Hit
CCCCGGAGGTGCCCTAGGAGCTAATTCACATCTTGGAAGTGCTCTAGGAA17270.17312085680288783No Hit
CTTCAAAATTAACCGCATGAGGGCTGTGCTTGCAGCAGGGTTTCACAAGA16860.16901086541382102No Hit
GCTTTAACGCGAGAACCACCGCCCTCTACCGGTGGGATCTGGAGGGACCT16750.1679081847972421No Hit
CTGCCATGCTGTCACTTTAGTGCAGGTACTTTAAGAAGGAAGACAAGCAG16750.1679081847972421No Hit
GAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCATGCTGTCAC16570.16610379833374933No Hit
GCCATGCTGTCACTTTAGTGCAGGTACTTTAAGAAGGAAGACAAGCAGGT16550.16590331094891683No Hit
GAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGGCGGTGGAGGGCGCCC16490.16530184879441923No Hit
TCCCTAGACTTATTGTGGTGATCGTTTTGCAGTGGATACAGATACCAAAT16470.1651013614095867No Hit
CAAGTAGAGAGGCAGGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAGCA15960.15998893309635726No Hit
GGATACAGCTGGGCAGGAAGATTATGACCGCTTGAGGCCTCTCTCCTATC15950.159888689403941No Hit
GATCTGGAGGGACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGC15930.15968820201910844No Hit
GGTCTGACAGGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGG15720.1575830844783669No Hit
ATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGTGCTGC15650.15688137863145307No Hit
TTAATAAACAGCACTTCAAAATTAACCGCATGAGGGCTGTGCTTGCAGCA15580.1561796727845392No Hit
CTACCGGTGGGATCTGGAGGGACCTGGAGTGACCAGGACCAGCAGCTGGC15540.15577869801487415No Hit
CCTCTAACCTCAGCTGTCCGTCAAGAGCAGCCGTTTCCCCTGTCCCGTAG15420.154575773705879No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC15000.15036553862439592No Hit
CTCTGGCCCTTTGCAGTGTTTGGACTTGAATTTGTGGGACATTAGGTCAA14840.14876163954573568No Hit
CTCTTGGGGCGGTGGAGGGCGCCCTGGCTTATCCTGAGGCTCCTAACCTG14740.14775920262157302No Hit
GGTATTAGTAGTAAGATGTTAGTGCAACCTTAATAACACGAAACAATCAT14640.1467567656974104No Hit
GTTTAGAAAATATCCCAGAAAAATGGACCCCGGAAGTCAAGCACTTCTGT14520.14555384138841523No Hit
CTTGAGGCCTCTCTCCTATCCGGACACTGATGTTATACTGATGTGTTTCT14470.14505262292633392No Hit
TCCCAGGACCTTGGAATCATGACCTGAGCTGAAGGCAGCGGCTTAACCCA14400.14435091707942008No Hit
GACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAAGGAGACCCCG14330.1436492112325062No Hit
GTTTGGACTTGAATTTGTGGGACATTAGGTCAAAGTGCCTTTTGAGACTC14230.14264677430834358No Hit
GTCAGAGACATTCACTTGTCACCTTCAAAACTCATCGGCTGCTTCGGGGA14200.1423460432310948No Hit
GTGGAGGGCGCCCTGGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAG14040.14074214415243455No Hit
GACAGGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGGCGGTG13900.13933873245860687No Hit
GGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGGCGGTGGAGG13700.1373338586102816No Hit
GCTGGATGCAGAGCTTCTAGTAAGTTAACGGTCAGAGACATTCACTTGTC13400.1343265478377937No Hit
CTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGTGCTGCTGTCTGGCT13320.13352459829846355No Hit
CATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATG13270.13302337983638224No Hit
CAATAAGGACATCACGTGGGAACAAGAGAGGACCGAGCTCAGACTTCAGA13120.1315197244501383No Hit
GTATTGAGGCTCGTCACAAGTATCCTATGTCAGTGACCATTTGTCTGCAT13030.1306175312183919No Hit
ACCTTACCACATTCTAAAGAATCAATGATTGTTTCGTGTTATTAAGGTTG12990.13021655644872687No Hit
CACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATGCAGCAGGAAC12760.1279109515231528No Hit
CTACGCACACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATGCAG12470.12500388444308114No Hit
GGATACAGATACCAAATCATTACGGTAGACACCAGAACTCATAGAAGGTT12300.12329974167200465No Hit
GTGTGAGGCCAGCCTGTGTCTGCGAGCTCCGGTTGGCCTGGAGTAACTGG12180.12209681736300947No Hit
GGCCTGGAGCCCTCAATAAGGACATCACGTGGGAACAAGAGAGGACCGAG12170.1219965736705932No Hit
CCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCAGCCTGTGTCTGC12150.12179608628576069No Hit
CTCTCACCCCGGAGGTGCCCTAGGAGCTAATTCACATCTTGGAAGTGCTC12130.12159559890092815No Hit
TCTTACTACTAATACCTGGTCAGCCTTCTCATGTTCACTTGTAGAAATGA12090.12119462413126311No Hit
GTCCCAACGTGCCCATCATCCTGGTTGGGAACAAGAAGGATCTTCGGAAT11900.11928999397535409No Hit
CTGGAAGGCTGATCCCTCTGGCCCTTTGCAGTGTTTGGACTTGAATTTGT11890.11918975028293784No Hit
GTGGGATACAGCTGGGCAGGAAGATTATGACCGCTTGAGGCCTCTCTCCT11850.11878877551327277No Hit
CCCTTGAGAGCAGATGTGTGAGGCCAGCCTGTGTCTGCGAGCTCCGGTTG11660.11688414535736376No Hit
GTCCCGTAGGGTATTGAGGCTCGTCACAAGTATCCTATGTCAGTGACCAT11560.11588170843320111No Hit
GTCTGGTGTGCTGCACACGGACAGCTGGATGCAGAGCTTCTAGTAAGTTA11420.11447829673937342No Hit
GACAAGTAGAGAGGCAGGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAG11390.11417756566212464No Hit
GCCCTGATAGTTTAGAAAATATCCCAGAAAAATGGACCCCGGAAGTCAAG10940.10966659950339276No Hit
ATCCCAGGACCTTGGAATCATGACCTGAGCTGAAGGCAGCGGCTTAACCC10870.10896489365647889No Hit
GGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAGCAGAGGTCCGGATGCG10580.10605782657640724No Hit
CTTGAGAGCAGATGTGTGAGGCCAGCCTGTGTCTGCGAGCTCCGGTTGGC10430.10455417119016328No Hit
CCAGTAGACGACCTTACCACATTCTAAAGAATCAATGATTGTTTCGTGTT10370.1039527090356657No Hit
ACGCTGAGCCTGTCCACGCCCCGGACACAATGATCAATTTCCGGAACACA10250.10274978472667054No Hit
TCTCTATAGAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCAT10180.10204807887975668No Hit
GAGTTGGCTTTGTGGGATACAGCTGGGCAGGAAGATTATGACCGCTTGAG10160.10184759149492416No Hit
CACTACCACTGTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGA10120.1014466167252591No Hit
GTGTCAGAAGGACCCCGGTGGTCAGGTGGGTTGGACATCATTAATAATCA10120.1014466167252591No Hit
CTGATGTTATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAA10040.100644667185929No Hit
TCTAGTAAGTTAACGGTCAGAGACATTCACTTGTCACCTTCAAAACTCAT10020.10044417980109646No Hit
GCACACGGACAGCTGGATGCAGAGCTTCTAGTAAGTTAACGGTCAGAGAC10000.10024369241626394No Hit
CATCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCT9990.10014344872384769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCTC100.007099237144.942738
TTTCGCT100.0071013677144.928197
TAGGGGC100.0071013677144.928197
CCTAAAT100.0071109603144.862761
AGCGAAA2550.0127.820091
GGATCGC259.367784E-487.04427145
GCGAAAG4000.081.473052
CGAAAGC4350.073.2565843
ATGGCGG300.001931837272.53689145
GAGCGAA300.001943024972.431391
GGTACGT300.001944185172.4204942
GAGAGTA5600.066.000719
TAGGAGA5600.065.977536
GAATTTA350.003559318662.17448145
AGGCGTA2100.062.0965046
GTACGTG350.00358132662.077823
TAGGCGT2150.060.6463135
ACAAGGT1700.059.6763157
GGCGTAG2200.059.2888077
GTAGGCG2200.059.2650154
Produced by FastQC (version 0.11.5)