FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588014
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG39410.24817161561547946No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT37660.23715156163610648No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG36330.22877632061178302No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT35770.22524990333838368No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA33490.21089234729668632No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA32710.2059805518087372No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG31090.19577913041068906No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG28700.18072888526171682No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC28640.1803510548395669No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA28530.17965836573229202No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG27170.1710942094968936No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC26250.16530080969059466No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC24880.15667368171817125No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG24700.15554019045172146No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC23150.14577957121284824No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT22800.14357556041697364No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC22190.13973428445844938No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG21990.13847484971794957No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT21950.13822296276984963No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG21810.1373413584514998No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21370.13457060202240032No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG21240.13375196944107545No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20550.12940691958635125No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20340.1280845131088265No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20320.12795856963477653No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20320.12795856963477653No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG19680.12392837846517726No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC19520.12292083067277744No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT18660.11750526128862845No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT18380.11574205265192875No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA17710.11152294627125453No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA17510.11026351153075475No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA17030.10724086815355532No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG16980.10692600946843038No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG16820.10591846167603057No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA16690.10509982909470571No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT16660.10491091388363076No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT16450.10358850740610599No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG16300.10264393135073116No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG16080.10125855313618143No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG16040.10100666618808148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG100.0071058795144.900095
ACAGACG203.8586612E-4108.80534145
AGTAGAA5250.096.593971
CAGGTAC3300.092.188813
GGTACTG3400.089.497115
GATCCCG259.368815E-487.04427145
GGCTGCC259.368815E-487.04427145
CCTTGTA259.422942E-486.918142
GCAGGTA3650.083.346172
CACCGTC754.8730726E-967.639227
GCACCGT754.8803486E-967.6264346
TTATAGG8800.067.506011
AGGTACT5000.063.7480054
GTAGAAA8550.062.701351
GCATTTA702.327215E-762.17448145
GACAGTA5350.060.9565737
AGCGAGC606.7869223E-660.447414145
ACCGTCT851.3094905E-859.685438
ACTCAGC851.31385605E-859.6609841
CCTTATA10900.057.8125272
Produced by FastQC (version 0.11.5)