FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999441
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGGGCAAGGCTAGGTCTGAAGTGTGAGCTCAGCGAGGAGCCTCCCA55320.5535094117611745No Hit
ATATATAACAGTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGTCCTCT52650.5267944781132653No Hit
CTATAATTACAGTAGCTTTGCCTACCCTAACATGAAAGGTTATGCTAGGG33430.3344869782208254No Hit
GAATATACCCACTTTAAAACTTCATGCATCTAACTAGCTTACTTATCTGA25230.25244111458305196No Hit
GCCCTAGTGTGTTCTGAATCGGGCAGTGATGGGACCTGACGTCCGTGCTC20560.20571499468202725No Hit
GTGCGGGCCACAGGGGGAATCTGGTTTTCCTCTTAGTGAGAGGAGAAGCC19760.19771052018078109No Hit
TCCCAACACTGCCTGAGGGTAGAGATGGCAATAATTACTGGTGGATCCGA18970.18980610161080044No Hit
CCTTAACTGTGCCTCCGGCCACCCCATCCTTTGCCTCAGTTGCTGGGCAA17040.170495306876544No Hit
CCATAGGAGGCATCAGGTATATTCTTGGGAAACAAAACATAGCAAAGTGA15180.1518849036611466No Hit
GTTCTGAATCGGGCAGTGATGGGACCTGACGTCCGTGCTCTGTGTAGGGC13650.13657634617751324No Hit
GTTATATAGATTATGAGACAAAGGATGGCCTCAGCTGCTCTGGAGGTTAG13650.13657634617751324No Hit
GTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGTCCTCTGCCATCCTTC13340.1334746123082803No Hit
AACCTGGACCGTACCCCCGACCCCAGCTCCTTAACTGTGCCTCCGGCCAC13280.13287427672068688No Hit
CCCCAGTGCTGTCCAGTTGTGTCACAAACTCACTACATGACTTCAGGGAA13160.13167360554549992No Hit
CTCCTTAACTGTGCCTCCGGCCACCCCATCCTTTGCCTCAGTTGCTGGGC13050.1305729903015786No Hit
GGTCTGAAGTGTGAGCTCAGCGAGGAGCCTCCCACAGGCATCTAGGCTGC12890.12897209540132934No Hit
CCCTTTATTCTTCTCTTTCTTTTCCTGGAACACAGCAAGTCCCAAGATCA12590.12597041746336202No Hit
TCTCTGTACTTTATAATTTGGATATCTTGGCCTCCCACTCAGAATATACC12480.12486980221944066No Hit
GGGTAGAGATGGCAATAATTACTGGTGGATCCGAGGTTGAACCCGATGGC12450.12456963442564395No Hit
TCATAAAGCCATCCAGCAGCTTCCTTGGAGAACCACGTTCACCCTGACCT12430.12436952256311279No Hit
GTAGTAGGTGGTGTCCGCACATCTGGCTGACTTTCACTGAAGATTGAGAG12360.12366913104425374No Hit
GCTCCTTAACTGTGCCTCCGGCCACCCCATCCTTTGCCTCAGTTGCTGGG12310.12316885138792585No Hit
GGTATATTCTTGGGAAACAAAACATAGCAAAGTGAGCATCAAATAACATT12090.12096762090008314No Hit
CTCTTATATATAACAGTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGT11990.11996706158742738No Hit
CTTATATATAACAGTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGTCC11530.11536448874921082No Hit
CTTTAAAACTTCATGCATCTAACTAGCTTACTTATCTGATTTATTGGCCT11300.11306320233010253No Hit
ATCCTAGGGAGATGTTATATAGATTATGAGACAAAGGATGGCCTCAGCTG11100.11106208370479098No Hit
GTCACACCCCGTGTCAGGCCCCTGGCTTTTGCTTCCAGACCTCTTAAATG10800.10806040576682364No Hit
TCCGTGCTCTGTGTAGGGCAAGGCTAGGTCTGAAGTGTGAGCTCAGCGAG10250.1025573295472169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG100.00708174145.06242145
AGCGAAA1950.0107.688761
GCGAAAG3100.067.746482
GCCGATC350.003573171862.1135527
AGCCGAT350.003576718762.0986
CGGAAAC702.3519169E-762.076243
CGAAAGC3550.059.1618583
CGTATCC400.00607781654.313992
CAGTCCA12750.053.4338079
ATATAAC14100.053.417853
AGACCCG701.7032458E-551.7483335
TATAACA14450.051.633144
TATATAA16350.050.493742
GCACTAC1900.049.5694546
ATATATA15700.048.8892331
AACAGTC14150.048.139837
GTTAGCC2700.045.633489
TAAGTTA2550.045.4573636
CTAAGTA803.7539383E-545.272994
CACTACG2100.044.859797
Produced by FastQC (version 0.11.5)