FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355400
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT32080.23668289803747972No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA21080.1555260439722591No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC16020.11819389110225764No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG15780.11642319610447101No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT13640.10063449904087354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4500.051.5271151
CGAAAGC6350.037.6563843
GCGAAAG6450.037.0643542
TATAGAC16100.031.0474172
ATAGACC16800.029.760363
TAGACCT18600.028.4396554
CGTTGAC1100.0096988226.3490943
GTACTAC3151.0404619E-925.3034951
GTATTAT6500.024.5249271
TATAGGG3551.5643309E-1024.4881022
GTATTAG5750.023.9433061
GTATATA21800.023.2669751
GTATTAC14150.023.043821
GTATAGG3503.5270205E-922.7731461
TATATAC24600.022.6805733
TATACAG13900.022.4173415
ATACTTA13550.021.9267866
CCTCTTA23750.021.6673288
CAGCTCG12800.021.5187159
TATACTT12900.021.3463845
Produced by FastQC (version 0.11.5)