FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960209
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1800.092.533541
CGAAAGC4950.039.5046043
TACACCG1952.624838E-729.7206045
GCGAAAG5650.029.4781762
CTATACG1050.00774530427.5919534
GCGGAGT1100.00964149626.380384145
TATACGG1100.00970790326.3432645
GTCTAAT5400.025.480251
ATTACCC4201.8189894E-1224.1417033
CCTATTC5850.023.5226843
CTTATAA1851.8447629E-423.4867741
TAAACCG1550.001818791423.3641554
CCCTATT5950.023.123732
TATAGAC5800.022.4732442
CTAATAT7150.022.2846473
TAATATC5900.022.0969454
CGGGTAT2404.3413358E-521.159267145
TCGTAAC2404.3799067E-521.1359
ATACCAT3809.116775E-920.9717186
CTATACT3851.0630174E-820.6939644
Produced by FastQC (version 0.11.5)