FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105944
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11660.1054302930347287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3600.038.2336041
CAGCTCG8300.025.326179
TATACAG9100.024.6804895
GCGAAAG6000.024.1420782
GTATTAC6700.023.787131
CTATATA3702.6375346E-1023.4938414
CGAAAGC6250.023.180593
TAGGCTA2252.6662738E-522.5397435
GTATATA15750.022.5377041
ATATATG9250.021.9275843
TATATGT10100.021.5166384
GTATAGG4058.1308826E-1021.464481
TATAGGG3809.151336E-920.965492
TATATAT9750.020.799332
ATACAGC10500.020.01436
TACTATA4001.6512786E-819.9172152
CTATACC4402.2882887E-919.7561844
TATATAC16200.019.6748833
GTACTAT5204.3655746E-1119.5037821
GTTATAT3351.324679E-619.4621961
Produced by FastQC (version 0.11.5)