FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197302
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG127361.063724941576979No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA109320.9130528471513453No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA100070.8357958142557184No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC90210.753443993244812No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG89190.744924839347132No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA88860.7421686424978827No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG84470.7055028722912013No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG82430.6884645644958415No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG77870.6503789353062135No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTCT77410.6465369639405931No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC73840.6167199252987133No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG68480.5719526067775715No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT58950.4923569826159148No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC58800.491104165866256No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT58400.4877633212004991No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT58340.48726219450063557No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC57140.4772396605033651No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG53800.44934360754429536No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG52480.43831882014729784No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG51570.430718398532701No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT51440.42963262401633No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG49120.41025572495494034No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA45810.38261023534580246No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC43480.36314981516776884No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC43210.360894745018383No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT39920.333416297642533No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA38200.31905066557977857No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC35130.29340968277009477No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC34990.29224038713707984No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAAGAGGCAATTCT34760.2903194014542697No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG34560.28864897912139126No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA34120.28497404998905873No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT32090.26801926331034276No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT31320.26158813732876085No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT30740.2567439125634134No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30470.2544888424140275No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30100.25139856109820247No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC29580.24705546303271855No Hit
GTTCATTGATGCTTCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC29200.24388166060024954No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC29150.24346405501702995No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG28800.24054081593449272No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG27930.23327447878647162No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG27710.23143701422030533No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA27130.2265927894549579No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG26870.22442124042221595No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC26320.21982757900680028No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT25870.21606912875782383No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT25850.215902086524536No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC25650.2142316641916576No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC25410.21222715739220346No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA25190.21038969282603723No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC25070.20938743942631016No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC24940.20830166490993918No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA24600.20546194694404585No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA24400.20379152461116745No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA24190.2020375811616451No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA23800.19878025761253218No Hit
CACTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCAAGATTGCTC23130.19318434279738944No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC22870.19101279376464753No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG22380.18692025904909537No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG22100.1845816677830656No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA21640.18073969641744522No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC21040.17572842941880998No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG20820.1738909648526437No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20130.16812800780421314No Hit
CTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAG19930.1664575854713347No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG19460.16253209298907043No Hit
CTTCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGA19330.16144631847269947No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG18510.1545975869078979No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG18250.152426037875156No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG18010.1504215310757019No Hit
GTATGATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGG17990.15025448884241402No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC17870.14925223544268698No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA17800.14866758762617954No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA17780.14850054539289168No Hit
GTATATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCG17760.14833350315960384No Hit
CATTTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTG17740.148166460926316No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC17630.14724772864323285No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG17600.14699716529330112No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA17520.14632899636014973No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA17330.14474209514391523No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC17290.14440801067733955No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA16880.14098364489493878No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT16720.13964730702863606No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT16680.13931322256206036No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA16410.1370581524126745No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCCTTCTCTCCTTTTGCAAG16140.13480308226328863No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC15880.1326315332305467No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC15800.1319633642973953No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA15680.13096111089766826No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA15670.13087758978102434No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA15510.1295412519147216No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT15320.1279543506984871No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA15170.12670153394882827No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT15070.12586632278238907No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC15060.12578280166574515No Hit
ATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATATGGGGAA14810.12369477374964712No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA14730.12302660481649576No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG14640.12227491476670047No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA14640.12227491476670047No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT14580.12177378806683693No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA14520.12127266136697341No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG14210.11868350675101186No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA14120.11793181670121658No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC14030.11718012665142127No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG14000.11692956330148951No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT13650.11400632421895228No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG13640.11392280310230836No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATA13530.11300407081922521No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCT13210.11033139508661975No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG13210.11033139508661975No Hit
GGATACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGAC13150.10983026838675622No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA13150.10983026838675622No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA12680.10590477590449193No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA12670.10582125478784803No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTCTCTTGTCTTCTTTGCCCAAA12450.10398379022168176No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT12360.10323210017188646No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG12320.10289801570531078No Hit
ATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAA12310.10281449458866686No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG12270.10248041012209116No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA12170.10164519895565197No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG12120.10122759337243235No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG12050.1006429455559249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGC100.0070840255145.04796145
CTCTATA100.0071017714144.926647
GCAGGTA13750.0118.4623952
CAGGTAC13850.0117.631643
AGCGAAA2900.0117.352221
GGTACTG14100.0114.033965
AGTAGAA15800.0109.529781
GACGCCC501.9463187E-10101.53358145
CCGCACG607.2759576E-1296.698654145
AGGTACT19050.087.049894
GAGTATA259.374872E-487.02878145
ACTGATC18850.086.117468
GAGCGAA608.385541E-1084.4769441
GTACTGA19650.082.583786
TACTGAT19950.081.725557
GGTCAAA16450.081.43241
AAATATA17400.077.421935
ATCTTAT1600.076.918282
GCGAAAG4650.076.285822
GTCAAAT17500.076.116792
Produced by FastQC (version 0.11.5)