FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836818
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAATGCAATCCAATCTTACAACCCTGAAATCACAATCCGAACCAAAA46020.5499403693515197No Hit
GTGTGGGACAGTGAAGAAACCGTCTTTCTTCCTCTTCTTACGGAATTCCT43290.5173167881188023No Hit
GTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAATGAGAG43030.5142097803823531No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG31640.37809894146636425No Hit
GTTGTAGGCAGTGTCTCCTGTGAGACCAAGGACCTGTTTGCTGCCCTGCC29810.35623038701366366No Hit
GGCCTAGAGGTGGGGGTCTCAGGCCTACGGAAGGTGACCTGAATGGGCAG28110.3359153364291877No Hit
GTGAAGAAACCGTCTTTCTTCCTCTTCTTACGGAATTCCTGTCCATCAAG28090.33567633583407624No Hit
GGTGTGGGACAGTGAAGAAACCGTCTTTCTTCCTCTTCTTACGGAATTCC26620.3181097920933823No Hit
GTAATGAGAGCTTGAAATAAACCCAAGGGCCTCAAATACCACACTAACAA24080.28775671651422413No Hit
GTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAATGAG23310.2785551936024321No Hit
CACTGATATGGCTCAGAGTCCGGCTGTAGGGGGCATGCCCATTGGCCAGG23020.2750896849733156No Hit
GCACAAGGAGTCCCAGGATGGTCTTGTGCAGGTGAGTCAGTACCATCAGA22830.2728191793197565No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC22730.2716241763441991No Hit
GTCCCAGGATGGTCTTGTGCAGGTGAGTCAGTACCATCAGAACCACACTT21720.2595546462910693No Hit
AAAGCAGGCAGTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGA21190.25322113052061496No Hit
GTCTTGTGCAGGTGAGTCAGTACCATCAGAACCACACTTCGGGAAGACTG21100.25214562784261335No Hit
GGCTAGGTCCCTGAGCTAGATTTTCTTGACCCAGAGCCTCCACTGAAGCC20250.24198810255037537No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA18430.2202390483952305No Hit
ACCCAGGTGCACCTGATTTCAGAACTTCTAAAATTAGCCTTTGCATCTTT17770.2123520287565516No Hit
GGACTGTAATGAGAGCTTGAAATAAACCCAAGGGCCTCAAATACCACACT17490.20900602042499086No Hit
GCTGAGCACGGATCCCAATGCAATCCAATCTTACAACCCTGAAATCACAA16940.2024335040594251No Hit
CTCTTCATCCACGGGCCTAGAGGTGGGGGTCTCAGGCCTACGGAAGGTGA16250.194187983528079No Hit
ATATTAATCTAAAGCATGAGAGAACTCTTAATTTTAATCATGACATTGTG15710.18773496746006896No Hit
GCAGTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAAT15210.18175995258228195No Hit
GATTATTATATATGTTTTGCGGGTGAGAGGAGAACAGAAAACTAAGGCTA14790.17674094008494082No Hit
GGCCAGGGCTGGGGCTTTGGCCCCCTCTTCATCCACGGGCCTAGAGGTGG14730.17602393829960636No Hit
TCAATGACCTCGGCACCATCAAGCTCAGCTTGGAAGTCACATGGAGCCCC14380.17184142788515544No Hit
GTAGGGGGCATGCCCATTGGCCAGGGCTGGGGCTTTGGCCCCCTCTTCAT14360.17160242729004394No Hit
CCTCTGGTAGTCCATCACAAGCCATGGAAGCCATCATGCCTTCCATTCCC14350.1714829269924882No Hit
AAATAAACCCAAGGGCCTCAAATACCACACTAACAAGTCAAAAATTAATT14080.1682564189584832No Hit
ATATAGTACAGTTTTTTCTCTTTTTTTGAGAGAGAGAGAAGAGAGAGCAG14070.16813691866092748No Hit
GGGCTGGGGCTTTGGCCCCCTCTTCATCCACGGGCCTAGAGGTGGGGGTC14040.16777841776826025No Hit
GATCTGGACTTGGGTTTTTTTTTGTTTTGTTTTGTTGTTGTTTGTTTGTT13990.16718091628048154No Hit
TGCTTCAGCAGTCCATTTCAGACTCCTCTGGTAGTCCATCACAAGCCATG13850.16550791211470117No Hit
ACTGTAATGAGAGCTTGAAATAAACCCAAGGGCCTCAAATACCACACTAA13790.1647909103293667No Hit
CTTATCTGACTGAGCCACCCAGGTGCACCTGATTTCAGAACTTCTAAAAT13160.15726239158335506No Hit
GTGCTGGAGCAGGTGCAGGTGCTGGAACAGGTGCAAGAGCAGGAACAGGA12970.15499188592979596No Hit
GGTTTAATGAGCATATTAACTTTAAAAAGGAATATCATTTAAATAGCAAA12960.15487238563224023No Hit
GTATATAGCAGGCAACTAAGGAAAAGCTAACTAGCCTGTATACAAAGGTT12810.15307988116890411No Hit
CTACATGCCAGGCAATATAGTACAGTTTTTTCTCTTTTTTTGAGAGAGAG12500.14937537194467615No Hit
AGCAGGGAGAGAGGGAAAGAAGCAGATCCCCTGCTGAGCACGGATCCCAA12400.14818036896911874No Hit
GGCAGTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAA12390.14806086867156298No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC12300.14698536599356132No Hit
GTGGTATGTCAAGCTAGTATTACTCAGGCAGAAGAACCAGCTGGAGCGAA12290.1468658656960056No Hit
GGCCTCACTGATATGGCTCAGAGTCCGGCTGTAGGGGGCATGCCCATTGG12010.14351985736444484No Hit
GAACTGAAGGGTCTGGCCAACCATGTGGTTGTAGGCAGTGTCTCCTGTGA11670.13945684724754964No Hit
TTCTTTATGAGACCTGGGCTTACATCGCAGCGTGTCTTCTTCACCATAAG11630.1389788460573267No Hit
GAAAGAAGCAGATCCCCTGCTGAGCACGGATCCCAATGCAATCCAATCTT11430.13658884010621186No Hit
GTCTTGAGGGCTGTAAATTGAAGAACTCCCTGAGGCCACAACGAGTTCAG11370.13587183832087743No Hit
GCTGTAGGGGGCATGCCCATTGGCCAGGGCTGGGGCTTTGGCCCCCTCTT11360.13575233802332168No Hit
GTATTACTCAGGCAGAAGAACCAGCTGGAGCGAAGGAAAGGCTGCAGAAG11330.13539383713065445No Hit
GATATGGCTCAGAGTCCGGCTGTAGGGGGCATGCCCATTGGCCAGGGCTG11240.1343183344526528No Hit
GTCCCAGGACCGTGGGATCATGACCTGAGCTGAAGGCAGAAGCTTAAACG11130.13300383117953962No Hit
GCCCCAAGTGGTAGCAGTGGACATCAATGACCTCGGCACCATCAAGCTCA11090.13252582998931667No Hit
AAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAATGGAATTTCTGAATGTA11040.13192832850153796No Hit
GCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCT10800.12906032136020018No Hit
AGTCAGTACCATCAGAACCACACTTCGGGAAGACTGATCTGGACTTGGGT10740.12834331957486572No Hit
GCCTAGAGGTGGGGGTCTCAGGCCTACGGAAGGTGACCTGAATGGGCAGA10740.12834331957486572No Hit
ATCCAATCTTACAACCCTGAAATCACAATCCGAACCAAAAGGCTTCAAGG10620.12690931600419686No Hit
CATCTGCACAGTGGCTAATGACTACGTCACGGACTTGTTAAAATTAAACA10470.12511681154086074No Hit
CTTTTATTGAGGGCTTACACTACATGCCAGGCAATATAGTACAGTTTTTT10440.12475831064819351No Hit
ATTTCAGACTCCTCTGGTAGTCCATCACAAGCCATGGAAGCCATCATGCC10280.12284630588730167No Hit
CTACAGCACAGCATGAATGTAACCTTCATATGCACTGAGAAACCAAAGTG10160.12141230231663278No Hit
ATATAGTATCATGTCATCTGCAGATAATGAAAGTTTTACTTCTTTCTTAT10150.12129280201907704No Hit
CGTCCACACTGGCCTCACTGATATGGCTCAGAGTCCGGCTGTAGGGGGCA10070.1203367996386311No Hit
GGCTCAGAGTCCGGCTGTAGGGGGCATGCCCATTGGCCAGGGCTGGGGCT9920.11854429517529498No Hit
CATCAGAACCACACTTCGGGAAGACTGATCTGGACTTGGGTTTTTTTTTG9910.11842479487773924No Hit
AAAGCAGGGAGAGAGGGAAAGAAGCAGATCCCCTGCTGAGCACGGATCCC9910.11842479487773924No Hit
CATTTAAATAGCAAAGAAATTTGACAATTGCAGTTACTTTTATTGAGGGC9880.11806629398507203No Hit
GCATGAATGTAACCTTCATATGCACTGAGAAACCAAAGTGTGTGACTCAC9860.11782729338996054No Hit
CCATTGGCCAGGGCTGGGGCTTTGGCCCCCTCTTCATCCACGGGCCTAGA9780.11687129100951461No Hit
AGCACAAGGAGTCCCAGGATGGTCTTGTGCAGGTGAGTCAGTACCATCAG9600.11472028565351128No Hit
ATAATATGCTGAGGCGTCAGGAGGAGCTGGAGAATGGGACAGCATGGTCC9430.11268878059506368No Hit
GGCTCAGGCTGCTGCACCAGAGGCCTGGGGGCTGAAGAGTGCCGGGCAGT9380.11209127910728497No Hit
GGCCTACGGAAGGTGACCTGAATGGGCAGAGGCTCAGGCTGCTGCACCAG9260.11065727553661608No Hit
GCCCTCAACTGCTTCCACTGTCAACAAGGCCTCCACAGTCACCAAGCGCT9190.1098207734537259No Hit
GGCTTACACTACATGCCAGGCAATATAGTACAGTTTTTTCTCTTTTTTTG9160.10946227256105867No Hit
GTACTATATTGCCTGGCATGTAGTGTAAGCCCTCAATAAAAGTAACTGCA8960.10707226660994384No Hit
GGATATCAAAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTG8910.10647476512216515No Hit
GTGCAGGAGCAGGGACAGGTGCTGGAGCAGGTGCAGGTGCTGGAACAGGT8910.10647476512216515No Hit
GCTAAGAACAGGAGAGGTAAAGGGCACGTTCTTTATGAGACCTGGGCTTA8890.10623576452705367No Hit
CTCAAATACCACACTAACAAGTCAAAAATTAATTTGATAAGCACAAGGAG8840.10563826303927497No Hit
CCACACTAACAAGTCAAAAATTAATTTGATAAGCACAAGGAGTCCCAGGA8780.10492126125394051No Hit
GGTAAAGGGCACGTTCTTTATGAGACCTGGGCTTACATCGCAGCGTGTCT8710.10408475917105034No Hit
TTACTTATCTGACTGAGCCACCCAGGTGCACCTGATTTCAGAACTTCTAA8680.10372625827838311No Hit
GTACCATCAGAACCACACTTCGGGAAGACTGATCTGGACTTGGGTTTTTT8620.10300925649304868No Hit
ATCTTACAACCCTGAAATCACAATCCGAACCAAAAGGCTTCAAGGCTTAC8600.10277025589793719No Hit
GATAAGCACAAGGAGTCCCAGGATGGTCTTGTGCAGGTGAGTCAGTACCA8570.10241175500526997No Hit
TCTTACAACCCTGAAATCACAATCCGAACCAAAAGGCTTCAAGGCTTACT8550.10217275441015848No Hit
GTTATAGAAACCGTTCTCAGGATTAACCTCGCTCACAGGTGAAAACTTTC8510.10169475321993551No Hit
ACCATGTGGTTGTAGGCAGTGTCTCCTGTGAGACCAAGGACCTGTTTGCT8510.10169475321993551No Hit
TCATGCTACTGTACACTTAACAGACTACAGCACAGCATGAATGTAACCTT8480.10133625232726828No Hit
CCCTGAGGCCACAACGAGTTCAGTGGGAAAAAGGACCATAAACGATTTGT8450.10097775143460108No Hit
GAATGGGCAGAGGCTCAGGCTGCTGCACCAGAGGCCTGGGGGCTGAAGAG8440.10085825113704534No Hit
GAACAGGAGAGGTAAAGGGCACGTTCTTTATGAGACCTGGGCTTACATCG8410.10049975024437811No Hit
CCTTCTAGGAAGTGGTTTAATGAGCATATTAACTTTAAAAAGGAATATCA8400.10038024994682238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT202.1454107E-6144.795621
CGTCGTT202.1460492E-6144.786972
AGCGAAA4450.0133.40721
CTCCTCG500.0130.57364145
GTATCCC203.8571662E-4108.81136145
TGAGTTC203.8792714E-4108.655175
TCGTTCT203.8811177E-4108.642174
GCGAAAG6500.092.440912
AATTCTA259.36519E-487.04909145
CGAAAGC7350.082.76513
GAAAGCA8550.071.1574554
GCTAGGT3850.063.9319082
ATAGGGG6450.061.7530253
CTAGGTC4250.061.343543
TAGGTCC4150.061.083964
GTGGCGA6650.059.9459729
TAGGGGT6750.059.0155034
ATACTCA400.006065712354.3405886
AATACTC400.006071456754.3275835
CAGTGTA7950.053.7902879
Produced by FastQC (version 0.11.5)