FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490349
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGAAAGTGGCCGGAGCGGGGGTAGGGCTGGTGGCCACCATGGTT12960.2643015484889334No Hit
CTTATAAAGTTTGTTGAATAAATGACCAAATTAGGCATGAAAAGATGATC7520.15336015776518358No Hit
ATTAAATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGC7430.15152473034512154No Hit
GGATAAAGAAAAGGATACGCAAAAGAAGCGAACGGAAATTTCAGAGATAA7130.14540663894491473No Hit
ATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGCTCCAG7100.14479482980489405No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG6850.13969642030472174No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC6630.1352098199445701No Hit
GTCTTTAATTGGGAAATGAAATATTGTTGTTAACTGCCCAACAACAGGTC6540.13337439252450806No Hit
CTTCAGATGGCTCCTTTTAAATTTCCAGTTGAACCACCCACATATTTTTT6390.13031534682440465No Hit
GATATGAACAATATTTGGTTCTAAAATGTGACTTGTCTTTAATTGGGAAA5690.11603980022392214No Hit
GTTGTGTATCTTCCCTTCTATTAAGTCTGTTTTCACATTCGCCGCCTTCT5590.11400043642385321No Hit
CTATTAAGTCTGTTTTCACATTCGCCGCCTTCTCCTTTCCCAAGAGAAGG5270.10747447226363263No Hit
TTCTATGACTAAAAAGTGATGAAATGCAACTAACCAGCTAGTCAGCTTTG5170.1054351084635637No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG5100.10400755380351545No Hit
TTCTAGTACTGAACAAATCTTGACTTTCTTTGGAAAAGCTGAACAAAAGA5080.10359968104350166No Hit
GCTCCAGTCCGTGCTATAGTCGGAACCACTGTGCAATCCTGCCAGCAAAT5010.10217212638345342No Hit
ATGCTAGACTATGTGATGTTCCAGCAGAGATCTGCTGAATAGCTATGCCA5010.10217212638345342No Hit
GAATATAAGAGAATACAAATAGGCTAGGTCATAAACGATCTTGTAAGTTA4960.10115244448341894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1650.0105.373571
CGAAAGC1950.089.144063
ATACCGA406.681811E-572.451726
GCGAAAG2550.068.168982
TCAATAC502.0189061E-457.943643
TAGTACT2600.052.9292834
CTAATAC553.2328587E-452.6760373
GAGTCGC752.5525467E-548.3208779
AGGCGTT450.00966161448.3011476
GGAACGT450.0096655148.2962235
TAATGCC450.00967330748.2863664
GGCTATA951.92904E-645.754311
CTAGTAC3000.043.457733
ACTCTAG1351.0311851E-842.9299741
GTATAAG1053.8403396E-641.3967631
ATATAAT3450.039.8968771
TATAATG2600.039.0005232
GTACTAA951.0327839E-438.1285931
GATGCCG1157.1478826E-637.8163389
AAACTCG1553.484456E-837.394446
Produced by FastQC (version 0.11.5)