FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_B08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_B08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267119
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC26920.21245044861611262No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG23490.18538116783033007No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA22310.17606870388653315No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT19970.1576016143708681No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18750.14797347368321365No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA17890.14118642369027692No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC17540.1384242521815236No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT15740.12421879870793508No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA15410.12161446557111054No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC15110.11924688999217911No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA14830.11703715278517646No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT14600.11522201150799569No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG14310.11293335511502867No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG13280.10480467896069746No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG12700.10022736617476338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG406.6863824E-572.450675
GACAGTA3600.062.4053237
TATACGT1206.002665E-1154.3337064
AGCGAAA2150.053.9125141
CAATTCG6350.050.2198839
AGTAGAA6450.048.2966231
GTACCGC657.359106E-444.5885436
GTAGAAA9200.042.5220341
CCTTATA8950.040.4640462
AAGTCCG951.03101855E-438.1454859
TTATACT9750.037.8942764
CGAAAGC3450.037.7958683
TATATTC7650.036.9312553
CCCTTAT10500.035.1875421
ATATTCA7000.035.1875424
TGTATAC2750.034.239942
AGGTCGT3850.031.9937296
ACAATTC10650.031.9836358
GCGGAAA3400.031.9610041
GAGTTAT3900.031.5897887
Produced by FastQC (version 0.11.5)