FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012059
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20050.19811097969584776No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19200.18971225985836793No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA17160.16955533224841635No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA16740.16540537656401455No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT15240.15058410626257956No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC14070.13902351542746025No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC13780.1361580698358495No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG12860.12706769071763602No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12280.1213367995344145No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA11990.11847135394280374No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11720.11580352528854543No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11560.1142225897897257No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11320.1118511865414961No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG11150.11017144257400013No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10640.10513221067151224No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10220.10098225498711044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2000.068.807791
AGTAGAA7300.055.5621721
CCTTATA7150.050.629812
GTAGAAA9250.050.1132131
GCGAAAG3050.047.475822
CAATTCG6150.045.9648369
CGAAAGC3200.043.0027433
TTATACT8350.042.5054974
TATATTC8350.040.766553
GTATAAT1303.7268364E-739.000371
TAGAAAC12350.038.105592
GGTACTG4850.035.848375
ATATTCA8600.035.3741764
ATATAGT6450.034.8109631
TTTAAAC8350.034.6949353
GACAGTA5550.033.952647
TATACTG12750.032.9547275
CAGGTAC5100.031.2424493
ACAATTC9450.030.6806378
GGTCAAA7600.030.496531
Produced by FastQC (version 0.11.5)