FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856713
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC31620.3690850961757321No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT23060.2691683212464384No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20250.23636853882221934No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC17940.20940501661583286No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA17560.20496945885028006No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA16430.19177951075797847No Hit
CTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA10760.12559631988775705No Hit
ATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTG9800.1143907002695185No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG9210.10750391321247607No Hit
GTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATT9070.10586976035148295No Hit
GGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGG8900.10388543187741986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.045.2836461
ATTACAT13650.039.2767073
GTATTAC13250.038.8243181
TTACATC14700.036.4712264
TATTACA15300.036.4573062
CACGCGC1251.2622953E-534.81037145
TATAGAC19150.034.802012
ATAGACC12250.033.7112433
TAGACCT12450.033.1696974
GAGCGAA2653.6379788E-1232.8092841
TACATCA16950.031.2061315
TAGACAG4600.029.9283315
ACATCAA17700.029.8873256
TACTGGG20800.029.6068063
ATAGTAC2701.72804E-1029.5165043
GCGAAAG4300.028.6445371
ATCAAAT17950.028.2648818
GATACTG22300.027.9418371
TATACGT1306.508593E-427.865234
ATACTGG22750.027.3843422
Produced by FastQC (version 0.11.5)