FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S3_R1_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S3_R1_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022993
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG10900.1065500936956558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.048.955171
CGAAAGC4800.039.219792
TATACGT1502.620618E-838.6315234
TTACGCA2201.6370905E-1136.2170524
GTATTAC7500.034.758171
TACGCAG2507.094059E-1131.8756855
GCGAAAG6550.028.7440321
TATAGAC8350.026.0140482
GTTACGC2808.285497E-925.8667933
TATATAC15100.025.4214063
TATTACA12600.024.7099062
CTAGTAC2351.3422086E-624.6560063
GTATATG3851.6370905E-1124.4510921
CAGCTCG7350.023.6658489
ATTACAT9900.023.4107533
GTATATA14600.023.3109861
TACGTGC2852.7941496E-722.8817636
ATATACT8050.022.4950664
TATACCA4850.022.405595
TATACTG3905.0749804E-1022.2906885
Produced by FastQC (version 0.11.5)