FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28822417
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2864720.9939208082375605No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA399930.1387565796442401No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG338930.11759249753412424No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC338070.11729411867158815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC82250.070.5038453
GCAGGTA84350.068.74882
GACAGTA94550.050.1861347
CAATTCG123550.047.751489
ACTGATC137650.044.0208178
CGAAAGC41950.043.8976633
CCTTATA168400.040.6108972
GGATAGC125150.038.8983845
CCCTTAT181950.037.7913131
GGTCGTT77100.036.7207577
TTTAAAC164300.036.5255363
AGGTCGT78250.036.461916
TATATTC143150.035.7674563
ATATAGT102700.029.116351
TCTAAGG82000.028.8864842
TATACTG318150.026.3347135
CTAAGGA94700.026.3088763
GTATGGA209550.025.5409831
TACTGGA289850.024.9923747
ACAAGGC167500.024.1029038
Produced by FastQC (version 0.11.5)