FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTATACCCTGTCATCTGCTAATAGATTCTATTCTATTTTCCTGAAGG15.0No Hit
GCGCAGTGCCCAGCACAGCCGCACAGAAGGAAGAAGGTGGGGGGCGCGGC15.0No Hit
GGGATTATGGATAACATGTCTTTACTAGAAGTGTCCTTTTCCTTCTAATG15.0No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC15.0No Hit
GCCTAGGGGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTG15.0No Hit
CAAACAGACTGTGTTCTTGAGGCTATGGCTTTCCTTGAAGAATCCCCCCC15.0No Hit
AACCGGGGCAGAATGAACGGTCTTTGCACATGGCTCATCTGCTTTCAGCC15.0No Hit
ATTTCACATTCAAAACCACCCCCTGTAATATGTAATGTTATTCCCATTTT15.0No Hit
AATCTCTAGAACTGCAACAGCATTTCAGAGATACCACCGAACCAACAATA15.0No Hit
GTTCATGATCCCAGGGTCCTGAGATCGAGTCCTACATAGGGCTCCCTGCT15.0No Hit
GTGCGGGGCTGCGCTGGCGGGAGTGACGGTCTCACCTTCTCATAACCGTT15.0No Hit
GTGCGCTGAGTTTAGGGGAATATGGTAAATGCCGCAACAGGAAGTTGCAC15.0No Hit
GCCCCCCAGTTGCCCACGAGGGCGCCGGGTAACCGCTTTCAGAGCCGTCC15.0No Hit
ATTAGAACCCAGGTCCCCCTCCGCCTAGCCCAGCATTTCTCCAACACCTA15.0No Hit
AAATAGAATTATGTAACCTAGTCATGAATCACTGTGGCATTTGCCAAGGC15.0No Hit
GGTGGAGTCATAGTTAAGGAAATGTAAAAGATTACTTATTACACGGTGAT15.0No Hit
AAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGAACTCC15.0No Hit
ACTCTTCAGTGTGCACTCACAGAGGAGCTGACAAACACACTAGTCTCCCT15.0No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT15.0No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)