FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACACCACC18.333333333333332No Hit
TCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCAT18.333333333333332No Hit
GTGGCAGTGAGTATTGACCGATTTTTAAGTGTTAGAGATCAAAGAGGTAA18.333333333333332No Hit
ACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTT18.333333333333332No Hit
AGTAGAACCAAGGCATTTTTTCATGAAGCACAAGTTAAATTCATTATTTT18.333333333333332No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACATCTACTTCAG18.333333333333332No Hit
TTGATGGCCATCCGATTTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGGAT18.333333333333332No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA18.333333333333332No Hit
CCCACAGTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGT18.333333333333332No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC18.333333333333332No Hit
GAGCAGGGCTAAGTGCATGGCAGGAATCAGGGTCATGAGTTACAAATTGA18.333333333333332No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)