FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50.3878975950349108No Hit
GTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATG40.3103180760279286No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.3103180760279286No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30.23273855702094648No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC30.23273855702094648No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.23273855702094648No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA30.23273855702094648No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG30.23273855702094648No Hit
TGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCACTGGA30.23273855702094648No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.23273855702094648No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC30.23273855702094648No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.23273855702094648No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.23273855702094648No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG30.23273855702094648No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG20.1551590380139643No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG20.1551590380139643No Hit
ATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGAA20.1551590380139643No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.1551590380139643No Hit
GGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATC20.1551590380139643No Hit
GAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCC20.1551590380139643No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20.1551590380139643No Hit
CACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACA20.1551590380139643No Hit
GGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACATTCGAGGATT20.1551590380139643No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT20.1551590380139643No Hit
AACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAG20.1551590380139643No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC20.1551590380139643No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA20.1551590380139643No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20.1551590380139643No Hit
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC20.1551590380139643No Hit
GCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATC20.1551590380139643No Hit
CTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATC20.1551590380139643No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA20.1551590380139643No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA20.1551590380139643No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG20.1551590380139643No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA20.1551590380139643No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC20.1551590380139643No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG20.1551590380139643No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.1551590380139643No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC20.1551590380139643No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.1551590380139643No Hit
TATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCA20.1551590380139643No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.1551590380139643No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA20.1551590380139643No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT20.1551590380139643No Hit
GGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAAT20.1551590380139643No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA20.1551590380139643No Hit
GTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAAT20.1551590380139643No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT20.1551590380139643No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG20.1551590380139643No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT20.1551590380139643No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT20.1551590380139643No Hit
GATCCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCAC20.1551590380139643No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT20.1551590380139643No Hit
CTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTTGAC20.1551590380139643No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGC20.1551590380139643No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.1551590380139643No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA20.1551590380139643No Hit
TCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACC20.1551590380139643No Hit
GTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGC20.1551590380139643No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCATCACCTT20.1551590380139643No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG20.1551590380139643No Hit
GTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTC20.1551590380139643No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT20.1551590380139643No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC20.1551590380139643No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT20.1551590380139643No Hit
ATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGA20.1551590380139643No Hit
GTGAAAATATGGCACCAGAAAAAGTAGACTTTGATGACTGCAAAGATGTT20.1551590380139643No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT20.1551590380139643No Hit
ATGTTCAATCGGGGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGC20.1551590380139643No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA20.1551590380139643No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.1551590380139643No Hit
GTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAAT20.1551590380139643No Hit
TAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCATGT20.1551590380139643No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.1551590380139643No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATCGAACAGGAACAGGATACA20.1551590380139643No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA20.1551590380139643No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG20.1551590380139643No Hit
CAGAGGAATCGTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGA20.1551590380139643No Hit
TCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCAT20.1551590380139643No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)