FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCCAGGACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGCTTTAA13.125No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC13.125No Hit
AATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTG13.125No Hit
GGGTTTGTTGGACCTCTAAACTACTTAGCCTCCAGGCCCCTCCTGGGATG13.125No Hit
GCGATATGTGCAAATGCAGACTAGTTGTGGGGAAGATATTGTCCTCCCAT13.125No Hit
GTCTTCGGAGTCTCGATACAGAATCATGGACAAAAGAAATACACAAAGAC13.125No Hit
AACGGAGCGCGAGCCGTACTTTGATGTCCTATAGGTGTTGAGGGATCCCG13.125No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACAATATC13.125No Hit
CTTAATCCTCATTAACAGTCCTCAGATGGTTTTATCATCTCCATTTTATG13.125No Hit
ATAAAACAAATTAGTAACAAATAGCCAAAATAAAAAATAACAAATAAACA13.125No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG13.125No Hit
GGCAGGTAGGAGACAAGGCCTGGAAATCGGCCACCATCCCTCAGAGCTAG13.125No Hit
ACACGGGCCTCCGAGTGAGAACTGGTGGACAGAGGACCTTCCGAGAGACT13.125No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT13.125No Hit
GGCATTGAGGAAAAGAGTCGCCTCTCCACTCCCGCTTGGCTCTGCGTCTA13.125No Hit
CCTGCCCCACCGAAGTCACCTGTCGATGCAGGTAGCAGTGTTCGTACTCG13.125No Hit
GAATAAAAGAAATGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA13.125No Hit
CTAGACACCCTGGCCTACCCCCTGCTAGCCAGTCACCCCGGGAGCCGATA13.125No Hit
AATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGG13.125No Hit
CTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCG13.125No Hit
CTCATGGAAGCCAGCCTGAGACTGAATTCAAGCATATGAAATGCCGAAAG13.125No Hit
CATAGTGAATGCACCAAACCATGAGGGAAAACAATCAGGAGTGGACAGAT13.125No Hit
ATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAA13.125No Hit
TACGTGATCCACGCAGCATAGGCAACTGAGTTCTTGTCCTCAAGATGCTC13.125No Hit
TCTTAGACCTGATAAGCAGCAGTCACAGCAGAGCCACAGTCACTGGGAAG13.125No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGACGATCAGGGT13.125No Hit
CCCGTGAATTCTATGAGATACATATTAATAGACCAGTTCTAAATATACGG13.125No Hit
CATATTTCCTTCTGCTGAAGGGAAAGCATTTGGATTTTACTTTTTCGAAA13.125No Hit
ATTAGATACAGAGGCGCTGTCTATGCGGACTCTGGAAATTTCAAACGATG13.125No Hit
GGATACACTCACAAGAGGCAATGTAAGCTTTCCGTAGAGTGTCTGAGCCT13.125No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG13.125No Hit
ACTGGGAGACGGGACTCGAAGCCAACAATCTATAATTAATCCTAGTGCCA13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)