FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATACAATGATCGTC13.4482758620689653No Hit
GAGGACTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAG13.4482758620689653No Hit
TTTCCATGCCCCATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCAAA13.4482758620689653No Hit
ATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCGAGCTGTGACTCTG13.4482758620689653No Hit
GGTCAACATCATCATTTCTCACTTCTCCTCCTGGAGTGTACAACTGCTCC13.4482758620689653No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGTTCAGCGTATTTAATGTCAG13.4482758620689653No Hit
GAAAATCGGTAATATCCGAAAATAGAAACTAACAAGTTTGCACCAAAATG13.4482758620689653No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATACAATTA13.4482758620689653No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG13.4482758620689653No Hit
TATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCAACAAGAGCAT13.4482758620689653No Hit
ATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCT13.4482758620689653No Hit
CAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTCCACCCCCGCTC13.4482758620689653No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGACCCCCGCACTAAGAAT13.4482758620689653No Hit
ACTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACAC13.4482758620689653No Hit
GTCTAAGGATGTCCACAAGCCTTACTCCTCCAATCTGTGTGCGGTGGCAC13.4482758620689653No Hit
GGAATCAACATGAGCAAAAAGAAGTCCTATATAAATAAGACAGGGACATT13.4482758620689653No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT13.4482758620689653No Hit
GGAGGATAGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATA13.4482758620689653No Hit
CCTGGGATGCAGAGTAGAGGTTTCGTATACTGTGTTGAAACTTTAGCTAG13.4482758620689653No Hit
CCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTAGAG13.4482758620689653No Hit
GTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTACT13.4482758620689653No Hit
GTTTTACTTTAGTCATTACAATGTACAGTTCAGTATAATCAGTGTTAAAC13.4482758620689653No Hit
CACAATCTGCTGAAGAGACTGAAGAAGGCACCGCATCATTTCCATGCCCC13.4482758620689653No Hit
TTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTC13.4482758620689653No Hit
TACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCA13.4482758620689653No Hit
GGTTGAGGATTAGCTCATCTTTCAGGTTTGGTGGGTTCACTTTCAAAAGG13.4482758620689653No Hit
CCCTTGCAGCGCGGCATCTGCACTCCCCTTCGCTGCTGGTAGGCCTCCAA13.4482758620689653No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13.4482758620689653No Hit
CAGCAGAAGTGTCCCACTGCAGGGCTACAGAATACAGAATGAAGGGAGTG13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)