FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTCCAGGAGAGAACTCCCACGAATTTATCTAATTTGTTGTTGGGCGCC14.0No Hit
GGCTTAGCCGGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT14.0No Hit
CACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATGCAGCAGGAAC14.0No Hit
GCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACT14.0No Hit
CCTCTGAGCAAACCCTGGAACCCAGCCCTGCTCGAACTTGAACAGAAGGT14.0No Hit
AAGCCACAGCTCGCAGAATGGCCTGTGTCCGACAGGTTCGGCAGTGTCTT14.0No Hit
GGGTCTGTATGGTCCGGTAAACCGTTCCTTGTTGAATGGGTGAGCACTGG14.0No Hit
TATTTATTTGACAGATGGAGATCACGAGTAGGCAGAGAGGCAGGCAGAGA14.0No Hit
AGTGAGGAAATGGTGGCAATACGGCACATTGTTTTTCACATTAAAACCCC14.0No Hit
GAGCAAGCAGCCCGATGCGGGACTCGATCCCAGGACCCTGAGATCATGAC14.0No Hit
GTAAACCAAAGTTAAGTTATTAAGTTCCGAAGCTCCTCCTTTACCTGGAA14.0No Hit
CACGAGGACTCTGTAGACACGAGGACCCTGTAGGCAGAGTTCCAAGGGGA14.0No Hit
TCCCAGGACCCCGGGATCACAACCCAAACTGAAGATTTAACCAACTGAGC14.0No Hit
GGGTGAGCATGGGCTGAGGGAAAGCTTCCTGCAGGGGTGAGATCTGAGGT14.0No Hit
TAGTGGGGTTGCCCAGAGGAGGCCCTTAAGGGTCAGCAGCTGGAATCCTC14.0No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC14.0No Hit
GGAAAGAGCCAGTTTGGGAACTAGGTGTCAGGAGTGAGGCCCACTGGGGT14.0No Hit
TAGGCAGTCTGCCCCATGTAACCTATCATATTGGTAGCTCCTGGAGGCTG14.0No Hit
GAGTATGGCTTCCTGACGGTGCCCAACTACGAGATGGAAGACCTGAAGCT14.0No Hit
CTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCG14.0No Hit
CACTTTGACTCAAGGAAGTTAGGTTCCACCCTCCCGAGCCACACGGTAAC14.0No Hit
GCCTCTGAGGCACCCCAGGAAATTTATTCTTAAAACAGTCCCAGTCCCAC14.0No Hit
GCTATAGGGTTCAGGATTACCTAATCTTTCATTTTTGTTGTGATCACTTT14.0No Hit
AGATGGGCGAGGCCGCCTCCCTTGCTGGAGAAGAAGGCGTGTGTCCCACA14.0No Hit
CCCTCAGACCTTGCATCTTCCTCAGGCTCCACCCTATACTGATGCTCCAC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)